Annexin V-FITC Apoptosis Detection Kit

For a Convenient, Easy & Fast method for Apoptosis Detection by Flow Cytometry and Fluorescence Microscopy

Annexin V-FITC Apoptosis Detection Kit are a convenient, easy-to-use and safe method for Apoptosis Detection. Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS).

Externalization of phosphatidylserine residues on the outer plasma membrane of apoptotic cells allows detection via Annexin V. Once the apoptotic cells are bound with labelled Annexin V, it can be visualized with fluorescent microscopy or cytometry.

Since loss of membrane integrity is a pathognomonic feature of necrotic cell death, necrotic cells will stain with specific membrane-impermeant nucleic acid dyes such as propidium iodide, the membrane integrity of apoptotic cells can be demonstrated by the exclusion of these dyes.

Price: 218.23 

SKU: CA011 Categories: ,

Detailed information:

Advantages & Features
  • Easy and one step protocol: get results with few handling steps.
  • Detection Method: Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy.
  • Mammalian Reactivity.
  • Proven performance for both adherent and suspension cells.
  • Versatile: most used kits availables.
  • Safe: non-enzymatic assay that avoids fixation.
Specifications
Includes

-500 µL Labeled Annexin V-FITC
-50 mL Binding Buffer (10x)
-1 mL Propidium Iodide

Download documentation
Datasheet
MSDS
Applications
  • Detect early/middle stages of Apoptosis.
  • Differentiate apoptosis from necrosis.
Tables & Figures

Quality Control
  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
Storage, Shipping & Guarantee
  • Shipped in: Gel pack.
  • Storage: All components should be stored at 4 ºC.
  • Recommendations: Protect from light and do not freeze.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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