Highly Efficient, Sccurate & Easy-to-use System compatible with Blunt and TA DNA Cloning
pSpark® Universal is a highly efficient, accurate and easy-to-use DNA Cloning kit ideal for a broad range of PCR fragments cloning applications. There is a range of DNA Polymerases available that do not generate PCR products with identical ends: proofreading DNA polymerases leave blunt ends while blends of polymerases and non-proofreading DNA polymerases leaves 3´A overhangs. Therefore, it is necessary to employ different vectors to clone both kinds of PCR fragments.
pSpark® Universal DNA cloning kit has been designed to save time, looking for a kit for several cloning scenarios. It is mainly composed of two cloning vectors which allow blunt or TA DNA cloning. For blunt DNA cloning and TA DNA cloning, pSpark® II DNA Cloning vector and pSpark® TA DNA Cloning vector, respectively, are included.