Proteinase K Solution, 20 mg/mL

Storage Buffer: 50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50%
Unit Definition:

Unit definition One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.

Includes for 5 mL:
– 5 x 1 mL Proteinase K Solution (20 mg/mL). Storage Buffer: 50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50%

• Isolation of genomic DNA from cultured cells and tissues.
• Removal of DNases and RNases during DNA and/or RNA purification.
• Determination of enzyme locations.

• Enzyme activity is assayed by digesting hemoglobin at a concentration of 16.7 mg/mL in a solution of 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl₂.
• Free of detectable RNase, DNase and exonuclease activities.

• Shipment at Ambient Temperature.
• Storage: 4 °C or -20 °C.
• Shelf Life: 24 months from the date of purchase, if it is properly stored.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.