2-O-Methyl Transferase
High Quality & Purity Enzyme manufactured from Recombinant source
Recombinant 2-O-Methyl Transferase from Vaccinia virus is produced in Escherichia coli. It catalyzes the addition of a methyl group at the 2′-O position of the first nucleotide adjacent to the cap structure at the 5′ end of the RNA. The enzyme utilizes SAM as a methyl donor to methylate capped RNA (Cap 0) resulting in a cap-1 structure.
GMP-grade is also available. Learn more
Detailed information:
Advantages & Features
- Isolated form a recombinant source.
- Extensivelly quality controlled: Endo/Exonucleases/RNAses free.
- Flexible Quality Systems that covers from RUO to cGMP.
Specifications
Protein information
Predicted MW: 39 kDa
Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie®blue staining.
Buffer Storage: 100 mM NaCl, 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1 % Triton®X-100.
Biological activity: One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.
Gene Information
Synonyms: mRNA (nucleoside-2′-)-methyltransferase; cap1-MTase
Specie: Bovine Vaccinia virus
GenBank accession number: AY243312.1
Protein RefSeq: P07617
Protein Family: Methyl transferase
Includes
– 2,000 U mRNA Cap 2′-O-Methyltransferase (50 U/µl)
– S-adenosylmethionine (SAM)
– Capping Buffer (10x)
Download documentation
Datasheet MSDSApplications
- 2´-O-methylation of capped in vitro transcripts helps the RNA evade innate immune response in vivo.
Tables & Figures
Quality Control
Quality Tests for Research Use Only version includes: Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, etc.
Advice
Avoid exposure to constant temperature changes.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
To be informed about our Safety Staments for GMP Products, please contact us.
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