CVX™ Poly(A) Tailing Kit
For a Verstatile, Easy and high-quality Polyadenylation of RNA
The Poly(A) Polymerase enzyme adds a chain of adenine nucleotides to the RNA. This process, called polyadenylation, increases the stability of RNA in eukaryotic cells and prevents its degradation.
The CVX™ Poly(A) Tailing kit is designed to add a poly(A) tail to the 3’ end of (m)RNA transcripts generated by in vitro transcription with CVX™ T7 ARCA (m)RNA Synthesis Kits. Then the resulting capped and tailed RNA can be used in transfection or micro-injection experiments where mRNA stability and translation efficiency may be increased relative to unmodified mRNA.
Detailed information:
Advantages & Features
- Quality Kit: High enzymatic purity, specificity, and activity.
- Verstatile: designed for use polyadenylated RNAs in a variety of applications.
- Easy and time-saving protocol with minimal handling steps.
Specifications
Includes
– 100 µl E. coli Poly(A) Polymerase (5 units/µl)
– 1 ml E. coli Poly(A) Polymerase Reaction Buffer (10X)
– 100 μl ATP (100 mM)
– 5 ml Nuclease-Free Water
Download documentation
DatasheetApplications
- Addition of a poly(A) tail to RNA synthesized in vitro in order to increase the stability.
- Addition of a poly(A) tail to an RNA molecule or a mixture of RNA molecules in order to provide a priming site for synthesis of first-strand cDNA using a primer with poly(dT) on its 3´ end.
- Synthesis of polyadenylated RNA for nucleic acid amplification methods or gene expression studies.
Tables & Figures
Quality Control
Advice
- Avoid freeze/thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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