CVX™ SP6 Transcription Kit
For an Easy, Scalable and High Yield in vitro RNA synthesis by SP6 RNA Polymerase
CVX™ SP6 Transcription Kit is designed for in vitro RNA synthesis by SP6 RNA Polymerase from linearized plasmid DNA templates or DNA templates generated by PCR. Synthesized RNA can subsequently be used for in vitro translation, RNase protection assays, RNA splicing, hybridization assays and Northern Blot experiments.
The kit contains sufficient reagents for 50 reactions of 20 μl each. A 20 μl reaction yields about 150 μg RNA with 1 μg of template added (1.5 kb RNA transcript). The SP6 RNA Polymerase Enzyme mix includes Ribonuclease Inhibitor Protein to protect newly transcribed RNA from degradation by RNases
Detailed information:
Advantages & Features
- High yield: produce microgram amounts of RNA from all templates.
- Scalable: increase the reaction size to produce milligram amounts of RNA.
- Convenient: Rnase inhibitor included.
Specifications
Includes
– 3 x 40 μl SP6 RNA Polymerase mix (Include RNase inhibitor)
– 200 μl SP6 Reaction Buffer 10 x (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM)
– 100 μl CTP (100 mM)
– 100 μl UTP (100 mM)
– 100 μl DTT (100 mM)
– 10 µl Control template 1 (200 ng/µl)
– 10 µl Control template 2 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
DatasheetApplications
- In vitro transcription.
- In vitro translation.
- Hybridization probes generation.
- RNase protection assays.
- RNA binding protein assays.
- Synthesis of antisense RNA.
Tables & Figures
Quality Control
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
- Avoid freeze/thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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