T7 Biotin16 RNA Labeling Kit
For Easy, Scalable & High-Quality RNA Synthesis with Modified Nucleotides
T7 Biotin16 RNA Labeling Kit is designed to generate randomly biotin-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments.
Biotin-16-UTP (Biotin-16-uridine-5’-triphosphate) is a biologically active analog of UTP (uridine 5′-triphosphate) that is readily incorporated into RNA during an in vitro transcription reaction by RNA Polymerases such as phage T7 RNA Polymerase.
Since T7 RNA polymerase does not significantly discriminate between the natural and the biotinylated nucleotide, the number of biotinylated positions per RNA transcript can be controlled by adjusting the ratio of the biotinylated nucleoside triphosphate (NTP) to the naturally occurring NTP in the transcription reaction. The biotin-labeled RNA can then be detected by a reporter molecule attached to streptavidin, avidin, or ant-biotin antibody.