T7 Digoxigenin RNA Labeling Kit
For Easy, Scalable & High-Quality RNA Synthesis with Modified Nucleotides
T7 Digoxigenin RNA Labeling Kit is designed to generate randomly Digoxigenin-modified RNA probes by in vitrotranscription with Dioxigenin-11-UTP.
Digoxigenin (DIG) is a steroid hapten derived from plants of the genus Digitalis (D. lanata, and the purple foxglove D. purpurea). It is widely used for generating non-radioactive probes and is an alternative to biotinylation.
Like biotin, DIG-labeled probes support both chromogenic and chemiluminescence detection formats. RNA probes are synthesized by in vitro transcription with DIG-11-UTP. These nucleotides are linked via a spacer arm to DIG. The resulting DIG-labeled molecules then function as hybridization probes in much the same manner as any other type of probe.