T7 Fluorescein RNA Labeling Kit
Easy, Convenient & High Quality Kit to generate randomly fluorescein-modified RNA Probes by In Vitro Transcription
T7 Fluorescein RNA Labeling Kit is designed to generate randomly fluorescein-modified RNA probes by in vitro transcription. Such probes are suited for in situ hybridization and Northern blot hybridization experiments.
The fluorescein labeled analog of uridine triphosphate (Fluorescein-12-UTP) is used for labeling RNA probes. Fluorescein-12-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP via in vitro transcription with RNA polymerases and optimized reaction buffer. fluorescein-modified RNA probe may be detected directly via fluorescence spectroscopy.
Detailed information:
Advantages & Features
- Quality Kit: High enzymatic purity, specificity, and activity.
- Convenient:Â Rnase inhibitor included.
- Easy and time-saving protocol with minimal handling steps.
Specifications
Includes
– 2 x 40 ÎĽl T7 RNA Polymerase mix (include RNase inhibitor)
– 200 ÎĽl T7 Reaction Buffer 10x (HEPES-based)
– 100 ÎĽl ATP (100 mM)
– 100 ÎĽl GTP (100 mM) 100 ÎĽl
– 100 ÎĽl CTP (100 mM) 100 ÎĽl
– 100 ÎĽl UTP (100 mM) 100 ÎĽl
– 10 ÎĽl Fluorescein-12-UTP (5mM)
– 100 ÎĽl DTT (100 mM)
– 10 µl Control template 1 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
Fluorescein-labeled RNA is used for hybridization to:
- in situ hybridization to tissues.
- Southern blots.
- plaque or colony lifts.
- RNase protection experiments.
Tables & Figures
Quality Control
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
- Avoid freeze/thaw cycles.
Storage, Shipping & Guarantee
- Shipped in:Â Gel Pack.
- Storage:Â -20 ÂşC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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