PickMutant™ Error Prone PCR Kit
For a Reliable, Robust & highly Efficient Random Mutagenesis by Error-Prone PCR
PickMutant™ Error-Prone PCR Kit is based on the protocol of Cadwell and Joyce (1992). epPCR takes advantage of the inherently low fidelity of Taq DNA Polymerase (with errors biased toward AT to GC changes), which may be further decreased by the addition of Mn2+, increasing the Mg2+ concentration, and using unequal dNTP concentrations. The rate of Mutagenesis achieved by error-prone PCR is in the range of 0.6-2.0 %.
Price: €240.79
Detailed information:
Advantages & Features
- Highly Efficient.
- Reliable.
- Robust.
Specifications
Includes
Includes for 30 rxn:
– 50 μL Taq Polymerase (5 U/μL)
– 200 μL 10x ep-PCR Buffer
– 80 μL ep-dNTP Mix (unbalanced dNTP ratio)
– 200 μL MnCl2 Solution (25mM)
– 2 x 1.5 mL Water (PCR Grade)
Download documentation
Applications
- Generation of a library of mutants.
- In vitro Molecular evolution.
Tables & Figures
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Quality Control
- Tested using the insert control DNA provided.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer) and Avoid freeze/thaw cycles.
- Shelf Life: 24 months from the date of purchase, if it is properly stored.
Citations
Khlusevich, Y., Matveev, A., Emelyanova, L., Goncharova, E., Golosova, N., Pereverzev, I., & Tikunova, N. (2022). New p35 (H3L) Epitope Involved in Vaccinia Virus Neutralization and Its Deimmunization. Viruses, 14(6), 1224.
Wong, T. S., & Tee, K. L. (2020). Gene Mutagenesis. In A Practical Guide to Protein Engineering (pp. 121-148). Springer, Cham.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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