Tetracycline Hydrochloride
Molecular biology grade Tetracycline Hydrochloride by Canvax, a widely used antibiotic for plasmid selection.
Tetracycline Hydrochloride is a broad-spectrum antibiotic active against Gram-negative and Gram-positive bacteria. It inhibits protein synthesis by binding the bacterial ribosome and blocking aminoacyl-tRNA binding at the A-site, which halts translation. Owing to its reliability and consistent performance, it is widely used for bacterial selection (Tetᴿ) and as a ligand in tetracycline-controlled gene expression systems (Tet-On/Tet-Off) in eukaryotic cells.
Canvax supplies Molecular Biology Grade Tetracycline Hydrochloride manufactured under stringent QC to ensure lot-to-lot consistency, high potency and excellent solubility profiles—ideal for cloning workflows, contamination control in cell culture, and inducible expression experiments.
For Research Use Only (RUO). Not for use in diagnostic procedures, human or veterinary applications.
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Price: 59.70 €
Detailed information:
Advantages & Features
- Proven performance in selection and inducible expression workflows.
- Consistent, lot-to-lot quality under stringent QC release criteria.
- High potency: ≥950 µg/mg (per current spec).
- Excellent solubility profile and light-sensitive handling guidance for reproducible results.
Specifications
- CAS Number: 64-75-5
- Chemical Formula: C22H24N2O8 · HCl
- Molecular Weight: 480.90 g/mol
- Appearance: Yellow powder
- Potency: >950 µg/mg
- Specific optical rotation: -240 to -255°
Includes
25 g Tetracycline Hydrochloride (powder)
Download documentation
Datasheet MSDSApplications
- Selection of Tetᴿ bacteria (plasmids with tetA/tetR markers). Typical final concentration: 10–20 µg/mL in LB/agar; titrate by strain.
- Tet-On/Tet-Off inducible expression in mammalian or yeast cells (optimize within 0.1–1 µg/mL range for expression level and cytotoxicity)
- Contamination control in cell culture workflows when appropriate. recommended concentration: 10 µg/mL.
Tables & Figures
Quality Control
- Strict quality controls in every manufacturing step to guarantee the highest quality.
Advice
- Light-sensitive: keep powder and solutions protected from light; avoid repeated warming cycles.
- Stock solutions (choose what fits your SOP):
- Water: readily soluble; prepare 10 mg/mL with gentle heating if needed; filter-sterilize 0.22 µm. Use promptly; monitor turbidity over time. protect from light.
- 70% Ethanol: common molecular biology stock 10–12 mg/mL; filter-sterilize; do not autoclave. Store aliquots at –20 °C, protected from light.
- Working concentrations:
- Bacterial selection: typically 10–20 µg/mL (LB broth/agar); titrate by strain/plasmid.
- Tet-On/Tet-Off: start 0.1–1 µg/mL tetracycline/doxycycline and optimize per cell line.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage (powder): +4 °C, tightly closed, desiccated and protected from light.
- Stock solutions: store aliquots at –20 °C, protected from light; avoid freeze-thaw.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. Handle as a hazardous chemical; avoid inhalation/ingestion/skin contact; use appropriate PPE and dispose according to local regulations. Light-sensitive. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
“Consistent potency and clean selections across multiple E. coli strains; no background colonies at 15 µg/mL. Good value.” — Italian Company
FAQs
Q1. What stock solvent should I use?
Tetracycline HCl is soluble in water and in alcohols. For cloning/selection, labs commonly prepare 10–12 mg/mL stocks in 70% ethanol or ~10 mg/mL in water; filter-sterilize and protect from light.
Q2. What working concentration do you recommend for bacterial selection?
Start with 10–20 µg/mL and titrate for your strain/plasmid.
Q3. Can I use it for Tet-On/Tet-Off induction?
Yes. For mammalian cells, optimize in the 0.1–1 µg/mL range (or use doxycycline as indicated by your system).
Q4. Can I autoclave tetracycline?
No. Prepare sterile stocks by filtration (0.22 µm).
Q5. How should I store the powder?
Per this product page: +4 °C; keep dry and protected from light.
Reviews