PBS (pH 7.4)
Sterile phosphate-buffered saline (PBS, pH 7.4) for research and manufacturing applications.
PBS (pH 7.4) is a phosphate-buffered saline solution formulated to maintain isotonic conditions in biological systems. It is commonly used for washing cells, diluting reagents, and sample preparation.
The buffer is ready to use and provides consistent pH stability, ensuring reliable results in research and routine laboratory workflows.
Have questions? Contact us
Price: 22.50 € – 1,332.10 €
Detailed information:
Advantages & Features
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High quality & sterile: 0.22 µm filtration ensures sterility.
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Reliable: reproducible results with batch-to-batch consistency.
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Versatile: compatible with immunoassays, Western blotting, and cell culture.
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Endotoxin controlled: <1 EU/mL.
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Scalable formats: 1 L, 5 L, 20 L, and 100 L available.
Specifications
Formula:
| Component Name | CAS | MW |
| Sodium Phosphate Dibasic | 7558-79-4 | 141.96 |
| Potassium Phosphate Monobasic | 7778-77-0 | 136.09 |
| Potassium Chloride | 7447-40-7 | 74.55 |
| Sodium Chloride | 7647-14-5 | 58.44 |
| WFI Quality Water | 7732-18-5 | 18.02 |
Standards Analytics (1x):
| Test | Specificitation | Method reference |
| Appearance | Clear and Colourless liquid | Ph. Eur. 2.2.1; 2.2.2 and 2.9.20. |
| pH | 7,2 – 7,6 | Potentiometry Ph Eur 2.2.3 |
| Osmolarity | 280 – 310 mOsm/kg | Ph. Eur. 2.2.35. |
| Bioburden | <0.1 CFU/mL | Ph. Eur. 2.6.12 |
| Endotoxin testing | < 1 EU/mL | Ph. Eur. 2.6.14 |
Includes
BR0003, BR0004 and BR0017:
– 1,000 mL PBS Solution (1X, 10X or 20X) in PET bottle.
BR0344:
– 5 L PBS Solution (1X) in 5L graduated square containers with moulded hand grip.
BR0347 and BR0348:
– 20 L PBS Solution (1X or 10X) in 20L graduated square containers with moulded hand grip.
BR0350:
– 100 L PBS Solution (1X) in single-use bag with plastic drum.
Download documentation
Datasheet MSDSApplications
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Cell culture manufacturing and maintenance.
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Upstream and downstream bioprocessing.
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Protein stabilization and dilution.
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ELISA, immunohistochemistry, Western blotting.
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Microbiology protocols.
Tables & Figures
Quality Control
Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:
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- Batch-to-batch consistency.
- Facilities audited regularly Quality audits.
- Lot specific CoAs and QA review previously to release a product.
- Material traceability, documented procedures and documented employee training.
- Equipment maintenance and monitoring records.
Advice
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Warm to room temperature before use to avoid precipitation.
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Do not freeze unless specified for custom formats.
Storage, Shipping & Guarantee
- Shipped at: Ambient temperature.
- Storage: Room temperature (15 – 25 ºC).
- Shelf life: 24 months from date of manufacturing.
Citations
- Low, Z. X., Kanauchi, O., AbuBakar, S., Tiong, V., & Hassandarvish, P. (2025). Protocol for Screening Host-Targeting Antivirals (HTAs) Using Human PBMCs and pDCs. Bio-protocol, 15(5), e5230.
- Onorini, D., Leonard, C. A., Phillips Campbell, R., Prähauser, B., Pesch, T., Schoborg, R. V., … & Borel, N. (2023). Neisseria gonorrhoeae Coinfection during Chlamydia muridarum Genital Latency Does Not Modulate Murine Vaginal Bacterial Shedding. Microbiology Spectrum, e04500-22.
- Fernandes, L. M. (2022). Análise da expressão de genes biomarcadores de bem-estar por hibridização in situ de ARN em paralarvas de cultivo de Octopus vulgaris (Cuvier, 1797) (Doctoral dissertation).
- Murillo, A., Cuenca, R., Serrano, E., Marga, G., Ahmed, A., Cervantes, S., … & Pastor, J. (2020). Leptospira Detection in Cats in Spain by Serology and Molecular Techniques. International Journal of Environmental Research and Public Health, 17(5), 1600.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO) and Further Manufacturing. The product was not tested for use in diagnostics, drug development, terapeutic use, nor is it suitable for direct administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website or Contact us.
Customers Review
Synonym(s)
Phosphate buffered saline, PBS buffer solution, Saline phosphate buffer, PBS solution pH 7.4, Isotonic buffer PBS
Also known as:
- Spanish: Tampón PBS pH 7.4, Solución salina fosfato, Buffer fosfato salino
- French: Tampon PBS pH 7.4, Solution saline phosphate, Tampon phosphate salin
- German: PBS-Puffer pH 7.4, Phosphatgepufferte Salzlösung, Isotoner Puffer
- Italian: Tampone PBS pH 7.4, Soluzione salina fosfato, Tampone fosfato salino
FAQs
Q: Does PBS contain calcium or magnesium?
A: No, it is formulated without Ca²⁺/Mg²⁺.
Q: What is the osmolarity?
A: 280–310 mOsm/kg.
Q: In what formats is PBS available?
A: From 1 L bottles to 100 L bags.
2 reviews for PBS (pH 7.4)
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Sarah Mitchell –
PBS is one of the reagents we consume most frequently in the lab, so consistency between batches is important. We use it routinely for washing steps in cell culture and immunoassay workflows, and it integrates perfectly into our standard procedures. The ready-to-use format saves preparation time during busy weeks.
Michael Turner –
We use this PBS mainly for routine cell washing and reagent dilution, and the ready-to-use format saves preparation time without adding extra handling steps. The pH range and osmolarity indicated in the product information fit well with our standard cell culture workflows, and the availability of larger formats is useful when several people in the lab are working with the same buffer.