PBS with Tween™ 20 (pH 7.4)

Sterile PBS with Tween™ 20 (pH 7.4) for reliable immunoassay wash and blocking.

The PBS with Tween™ 20 (pH 7.4) is a sterile, ready-to-use buffer widely used in immunoassays such as ELISA and Western blotting. The addition of Tween™ 20, a non-ionic detergent, minimizes non-specific binding and background staining, ensuring clearer and more reliable results.

Supplied in convenient 1× and 10× formats, this molecular biology–grade buffer provides consistent performance, reproducibility, and compatibility across a wide range of protein analysis techniques.

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Product available under ISO13485 / cGMP or with custom formulation, packaging or application. Learn more >

Price: 45.68 249.00 

SKU: BR0008 Categories: ,

Detailed information:

Advantages & Features
  • Reduces non-specific binding and protein–protein interactions.

  • Sterile-filtered (0.22 µm) to ensure contamination-free use.

  • Proven performance in ELISA and Western blotting.

  • Ready-to-use in 1× or concentrate 10× formats.

  • High quality: DNase-, RNase-, and protease-free.

Specifications
  • Format: Liquid
  • Composition (1X): 0.01 M PBS, 0.138 M NaCl, 0.0027 M KCl and 0.05% Tween™ 20
  • Direction of use (1x): Ready-to-use solution; (10X): Dilute 10x solutions 10:1 to make a 1x working solution.
  • Sterilization: purified using a 0.22 µm filtration process [aseptically filled]
Includes
  • Includes for 1L: 1,000 mL PBS with Tween™ 20 (pH 7.4)
  • Includes for 5L: 5 L PBS with Tween™ 20 (pH 7.4) in 5L graduated square containers with moulded hand grip.
Download documentation
Datasheet
MSDS
Applications
  • Wash buffer in ELISA and Western blot assays.

  • Blocking solution for immunoassays.

  • Protein plate coating.

  • General laboratory washing buffer.

Tables & Figures

Quality Control
  • Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:
      • Batch-to-batch consistency.
      • Facilities audited regularly Quality audits.
      • Lot specific CoAs and QA review previously to release a product.
      • Material traceability, documented procedures and documented employee training.
      • Equipment maintenance and monitoring records.

    Request a Certificate of Analysis (CoA)

Advice
  • Dilute 10× concentrate 1:10 before use.

  • Store tightly closed to avoid contamination.

Storage, Shipping & Guarantee
  • Shipped at: Ambient Temperature.
  • Storage: Room Temperature.
  • Recommendations: Store the product in a dry place.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review
FAQs

Q: What is the composition of PBS-T?
A: 0.01 M PBS, 0.138 M NaCl, 0.0027 M KCl, 0.05% Tween™ 20.

Q: In what formats is it supplied?
A: 1 L, 5 L, and 20 L in 1× or 10× concentrations.

Q: Is it suitable for ELISA?
A: Yes, ideal as wash buffer and to reduce background signals.

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