PBS (pH 7.4)

Name: PBS (pH 7.4)
SKU: BR0003
Availability: InStock

Sterile phosphate-buffered saline (PBS, pH 7.4) for research and manufacturing applications.

PBS (pH 7.4) is a phosphate-buffered saline solution formulated to maintain isotonic conditions in biological systems. It is commonly used for washing cells, diluting reagents, and sample preparation.

The buffer is ready to use and provides consistent pH stability, ensuring reliable results in research and routine laboratory workflows.

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Price: 21.92 € – 1,298.23 €

size

1 L (10X)

1 L (20X)

1 L (1X)

5 L (1X)

20 L (1X)

20 L (10X)

100 L (1X)

SKU: BR0003 Categories: Saline Buffers, Buffers & Solutions

Detailed information:

Advantages & Features

High quality & sterile: 0.22 µm filtration ensures sterility.
Reliable: reproducible results with batch-to-batch consistency.
Versatile: compatible with immunoassays, Western blotting, and cell culture.
Endotoxin controlled: <1 EU/mL.
Scalable formats: 1 L, 5 L, 20 L, and 100 L available.

Specifications

Formula:

Component Name	CAS	MW
Sodium Phosphate Dibasic	7558-79-4	141.96
Potassium Phosphate Monobasic	7778-77-0	136.09
Potassium Chloride	7447-40-7	74.55
Sodium Chloride	7647-14-5	58.44
WFI Quality Water	7732-18-5	18.02

Standards Analytics (1x):

Test	Specificitation	Method reference
Appearance	Clear and Colourless liquid	Ph. Eur. 2.2.1; 2.2.2 and 2.9.20.
pH	7,2 – 7,6	Potentiometry Ph Eur 2.2.3
Osmolarity	280 – 310 mOsm/kg	Ph. Eur. 2.2.35.
Bioburden	<0.1 CFU/mL	Ph. Eur. 2.6.12
Endotoxin testing	< 1 EU/mL	Ph. Eur. 2.6.14

Includes

BR0003, BR0004 and BR0017:
– 1,000 mL PBS Solution (1X, 10X or 20X) in PET bottle.

BR0344:
– 5 L PBS Solution (1X) in 5L graduated square containers with moulded hand grip.

BR0347 and BR0348:
– 20 L PBS Solution (1X or 10X) in 20L graduated square containers with moulded hand grip.

BR0350:
– 100 L PBS Solution (1X) in single-use bag with plastic drum.

Download documentation

Datasheet

MSDS

Applications

Cell culture manufacturing and maintenance.
Upstream and downstream bioprocessing.
Protein stabilization and dilution.
ELISA, immunohistochemistry, Western blotting.
Microbiology protocols.

Tables & Figures

Quality Control

Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:

- Batch-to-batch consistency.
- Facilities audited regularly Quality audits.
- Lot specific CoAs and QA review previously to release a product.
- Material traceability, documented procedures and documented employee training.
- Equipment maintenance and monitoring records.

Request a Certificate of Analysis (CoA)

Advice

Warm to room temperature before use to avoid precipitation.
Do not freeze unless specified for custom formats.

Storage, Shipping & Guarantee

Shipped at: Ambient temperature.
Storage: Room temperature (15 – 25 ºC).
Shelf life: 24 months from date of manufacturing.

Citations

Low, Z. X., Kanauchi, O., AbuBakar, S., Tiong, V., & Hassandarvish, P. (2025). Protocol for Screening Host-Targeting Antivirals (HTAs) Using Human PBMCs and pDCs. Bio-protocol, 15(5), e5230.
Onorini, D., Leonard, C. A., Phillips Campbell, R., Prähauser, B., Pesch, T., Schoborg, R. V., … & Borel, N. (2023). Neisseria gonorrhoeae Coinfection during Chlamydia muridarum Genital Latency Does Not Modulate Murine Vaginal Bacterial Shedding. Microbiology Spectrum, e04500-22.
Fernandes, L. M. (2022). Análise da expressão de genes biomarcadores de bem-estar por hibridização in situ de ARN em paralarvas de cultivo de Octopus vulgaris (Cuvier, 1797) (Doctoral dissertation).
Murillo, A., Cuenca, R., Serrano, E., Marga, G., Ahmed, A., Cervantes, S., … & Pastor, J. (2020). Leptospira Detection in Cats in Spain by Serology and Molecular Techniques. International Journal of Environmental Research and Public Health, 17(5), 1600.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO) and Further Manufacturing. The product was not tested for use in diagnostics, drug development, terapeutic use, nor is it suitable for direct administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website or Contact us.