1M Sodium Chloride, 50mM Sodium Phosphate, 2mM EDTA Buffer – Canvax

ELISA Wash Buffer

Name: ELISA Wash Buffer
SKU: BR0390
Availability: InStock

Sterile, nuclease-free buffer (pH 7.0) with NaCl, sodium phosphate & EDTA for molecular biology and biomanufacturing.

ELISA Wash Buffer, composed of 1 M Sodium Chloride, 50 mM Sodium Phosphate, and 2 mM EDTA (pH 7.0), is a sterile, ready-to-use, high-purity solution specifically optimized for enzyme-linked immunosorbent assays (ELISA) and other molecular biology applications. Its balanced formulation provides ionic strength, pH stabilization, and protection against metal ion–dependent degradation, ensuring accurate and reproducible assay results.

This buffer ensures consistent washing performance, effectively removes unbound reagents, and minimizes background signal to enhance assay sensitivity and precision. Sodium chloride maintains ionic strength, sodium phosphate stabilizes the pH during wash cycles, and EDTA chelates divalent cations to protect proteins and nucleic acids from degradation.

Produced in an ISO 13485 and GMP-certified facility under ISO 9001 guidelines, the buffer offers nuclease-free quality, batch-to-batch consistency, and contamination-free performance. Its reliability and stability make it ideal for diagnostic ELISA testing, HPLC workflows, cell culture, and nucleic acid stabilization in both research and routine laboratory environments.

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Price: 65.00 € – 260.00 €

SKU: BR0390 Categories: Sodium Buffers, Buffers & Solutions

Detailed information:

Advantages & Features

Sterile and nuclease-free: DNase, RNase, and protease not detected.
Balanced composition: ionic strength, pH stability, and chelation.
Convenient: ready-to-use solution.
Versatile: suitable for molecular biology and biomanufacturing.

Specifications

Format: Liquid, Ready-to-use solution
Composition: Sodium Chloride (~6%); Sodium Phosphate (Monobasic) (< 1%); Sodium Phosphate (Dibasic) (<1%); EDTA (<1%) and WFI Water up to rest.
Sterilization: purified using a 0.22 µm filtration process [aseptically filled]

Includes

Includes for 1L: 1,000 mL solution in PET Bottle
Includes for 5L: 5L solution in 5L graduated square containers with moulded hand grip.

Download documentation

Datasheet

MSDS

Applications

Designed for the most commonly used Research and Biomanufacturing applications, such as:

HPLC workflows.
Cell culture supplement.
DNA/RNA stabilization.
Buffer preparation.
Biomanufacturing processes.

Tables & Figures

Quality Control

Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:

- Batch-to-batch consistency.
- Facilities audited regularly Quality audits.
- Lot specific CoAs and QA review previously to release a product.
- Material traceability, documented procedures and documented employee training.
- Equipment maintenance and monitoring records.

Request a Certificate of Analysis (CoA)

Advice

Mix gently before use.
Use aseptic technique to avoid contamination.

Storage, Shipping & Guarantee

Shipping: Ambient Temperature.
Storage: Room Temperature or at 4 ºC.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.