Tris-Glycine Buffer (pH 8.3)
Premeasured Tris-Glycine pouch (pH 8.3) for fast and consistent electrophoresis buffer preparation.
Tris-Glycine Buffer (pH 8.3) provides a quick and consistent way to prepare electrophoresis and transfer buffer with precise pH and ionic strength. Each pouch contains the exact formulation required to make 1 L of 1× buffer, ensuring reliable and reproducible results for protein separation and blotting.
The premeasured pouch format eliminates weighing and pH adjustment steps, saving time and reducing preparation errors. Ideal for PAGE electrophoresis, Western blotting, and protein transfer, it guarantees consistent buffer conditions between runs.
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Price: 72.42 €
Detailed information:
Advantages & Features
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Premeasured format: Each pouch prepares 1 L of 1× buffer.
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Accurate composition: Ensures reproducible pH and ionic strength.
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Fast preparation: Dissolves completely in minutes.
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Consistent performance: Provides stable migration and transfer conditions.
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Clean handling: Dust-free powder for precise preparation.
Specifications
| Parameter | Specification |
|---|---|
| Format | Pouch |
| Composition | 25 mM Tris, 192 mM Glycine |
| pH | 8.3 ± 0.05 (1× solution at 25 °C) |
| Concentration | 1× when dissolved in 1 L of water |
| Appearance | White, free-flowing powder |
| Solubility | Complete in <5 min at room temperature |
Includes
Includes for 5L:
– 5 Exactly pre-weighed pouchs (1,000 mL/pouch)
Download documentation
Datasheet MSDSApplications
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PAGE electrophoresis running buffer.
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Western blot transfer buffer.
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Protein separation and analysis.
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Molecular biology and biochemistry workflows.
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General electrophoresis applications.
Tables & Figures
Quality Control
- Each batch is tested for pH, conductivity, and solubility to ensure reproducible electrophoretic performance and consistent buffer quality.
Advice
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Pour one pouch into 1 L of deionized water.
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Stir gently until fully dissolved; do not adjust pH.
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Use freshly prepared buffer for optimal results in electrophoresis and transfer.
Storage, Shipping & Guarantee
- Shipped at: Room temperature.
- Storage: Room temperature.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What is the final composition of the 1× buffer?
25 mM Tris and 192 mM Glycine at pH 8.3.
Q2: Can it be used for both electrophoresis and Western blotting?
Yes. It provides stable ionic conditions for protein migration and transfer.
Q3: Does the buffer require pH adjustment?
No. Each pouch provides the correct pH and ionic balance when dissolved in 1 L of water.
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