Tris-Glycine SDS (pH 8.3)

Premeasured Tris-Glycine-SDS pouch (pH 8.3) for fast and consistent electrophoresis buffer preparation.

Tris-Glycine-SDS Buffer (pH 8.3) provides a fast and reproducible way to prepare electrophoresis running buffer. Each pouch contains the exact formulation required to make 1 L of 1× buffer, ensuring consistent ionic strength and pH for reliable protein separation in SDS-PAGE.

The premeasured pouch eliminates weighing errors and simplifies preparation, delivering high reproducibility between gels. It’s ideal for polyacrylamide gel electrophoresis (PAGE) and Western blotting, providing stable migration conditions and clear, well-resolved protein bands.

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Price: 71.30 

SKU: BR0052-XL Categories: ,

Detailed information:

Advantages & Features
  • Premeasured format: Each pouch prepares 1 L of 1× buffer.

  • Consistent performance: Reproducible pH and ionic strength for reliable separations.

  • Ready in minutes: Dissolves quickly without pH adjustment.

  • Accurate and clean: Minimizes preparation errors and contamination.

  • Stable dry format: Long shelf-life for routine electrophoresis use.

Specifications
Parameter Specification
Format Pouch
Composition 25 mM Tris, 192 mM Glycine, 0.1% SDS
pH 8.3 ± 0.05 (1× solution at 25 °C)
Concentration 1× when dissolved in 1 L of water
Appearance White, free-flowing powder
Solubility Complete in <5 min at room temperature
Includes

Tablets format – Includes for pouch:
– Exactly pre-weighed powder (1,000 mL/Pouch)

Download documentation
Datasheet
MSDS
Applications
  • SDS-PAGE electrophoresis.

  • Western blot transfer.

  • Protein separation and analysis.

  • Biochemical and molecular biology workflows.

  • General laboratory electrophoresis applications.

Tables & Figures

Quality Control
  • Each lot is tested for pH accuracy, conductivity, and solubility to ensure consistent electrophoretic performance and reproducibility between runs.
Advice
  • Pour the contents of one pouch into 1 L of deionized water.

  • Mix until completely dissolved; do not adjust pH.

  • Use freshly prepared buffer for optimal electrophoresis results.

Storage, Shipping & Guarantee
  • Shipped at: Ambient Temperature.
  • Storage: Room temperature.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review
FAQs

Q1: What is the final composition of the 1× buffer?
25 mM Tris, 192 mM Glycine, and 0.1% SDS at pH 8.3.

Q2: Can it be used for Western blot transfer?
Yes. It provides stable ionic conditions suitable for protein transfer to membranes.

Q3: Does the buffer require pH adjustment?
No. Each pouch yields the correct pH and concentration when dissolved in 1 L of water.

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