Annexin V-FITC Apoptosis Detection Kit

-500 µL Labeled Annexin V-FITC
-50 mL Binding Buffer (10x)
-1 mL Propidium Iodide

• Early apoptosis detection in mammalian cells.

• Cancer research and drug screening.

• Immunology and toxicology studies.

• Flow cytometry and fluorescence microscopy assays.

• Each lot is validated for FITC fluorescence intensity, Annexin V binding activity, and performance in both flow cytometry and microscopy platforms.

• Protect FITC-conjugated reagents from light to prevent signal loss.

• Use PI or 7-AAD for multiparametric discrimination of apoptosis and necrosis.

• Process freshly harvested cells for best results.

• Shipped in: Gel pack.
• Storage: All components should be stored at 4 ºC.
• Recommendations: Protect from light and do not freeze.

• Ganci, D., D’Anna, L., Abruscato, G., Le Chevalier, M., Quideau, O., Cataldo, S., … & Bonsignore, R. (2025). Harnessing redox reactions for anticancer effects: A copper (II) Schiff base complex induces apoptosis in HepG2 liver cancer cells via ROS generation. Journal of Inorganic Biochemistry, 112938.
• García, J. M. S. (2023). Estudio del efecto del tratamiento con pravastatina sobre la función endotelial vascular en mujeres con alto riesgo de preeclampsia tardía (Doctoral dissertation, Universidad de Murcia).
• Lagal, D. J., López-Grueso, M. J., Pedrajas, J. R., Leto, T. L., Bárcena, J. A., Requejo-Aguilar, R., & Padilla, C. A. (2023). Loss of PRDX6 Aborts Proliferative and Migratory Signaling in Hepatocarcinoma Cell Lines. Antioxidants, 12(6), 1153.
• Punginelli, D., Catania, V., Abruscato, G., Luparello, C., Vazzana, M., Mauro, M., … & Schillaci, D. (2023). New Bioactive Peptides from the Mediterranean Seagrass Posidonia oceanica (L.) Delile and Their Impact on Antimicrobial Activity and Apoptosis of Human Cancer Cells. International Journal of Molecular Sciences, 24(6), 5650.
• Carrión-Marchante, R., Pinto-Díez, C., Klett-Mingo, J. I., Palacios, E., Barragán-Usero, M., Pérez-Morgado, M. I., … & Martín, M. E. (2023). An Aptamer against MNK1 for Non-Small Cell Lung Cancer Treatment. Pharmaceutics, 15(4), 1273.
• Vašícek, J., Baláži, A., Svoradová, A., Vozaf, J., Dujícková, L., Makarevich, A. V., … & Chrenek, P. Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers. Int. J. Mol. Sci.
• Tirpák, F., Halo, M., Tokárová, K., Binkowski, L. J., Vašíček, J., Svoradová, A., … & Massányi, P. (2021). Composition of Stallion Seminal Plasma and Its Impact on Oxidative Stress Markers and Spermatozoa Quality. Life, 11(11), 1238.
• Křen, V. Antioxidative effect of dietary flavonoid isoquercitrin on human ovarian granulosa.
• Luparello, C., Ragona, D., Asaro, D. M. L., Lazzara, V., Affranchi, F., Arizza, V., & Vazzana, M. (2020). Cell-Free Coelomic Fluid Extracts of the Sea Urchin Arbacia lixula Impair Mitochondrial Potential and Cell Cycle Distribution and Stimulate Reactive Oxygen Species Production and Autophagic Activity in Triple-Negative MDA-MB231 Breast Cancer Cells. Journal of Marine Science and Engineering, 8(4), 261.
• José, M., Grueso, L., María, R., Valero, T., Beatriz, C. H., Daniel, L. R., … & Alicia, P. C. (2019). Peroxiredoxin 6 Down-Regulation Induces Metabolic Remodeling and Cell Cycle Arrest in HepG2 Cells. Antioxidants, 8(11), 505.
• Luparello, C., Ragona, D., Asaro, D. M. L., Lazzara, V., Affranchi, F., Celi, M., … & Vazzana, M. (2019). Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells. Biology, 8(4), 76.

• Vašíček, J., Baláži, A., & Chrenek, P. (2019). STAUROSPORINE-INDUCED APOPTOSIS ANALYSED BY LOW-COST AND HIGH-COST ANNEXIN V ASSAYS. Slovak Journal of Animal Science, 52(02), 47-52.

• García-Pastor, C., Blázquez-Serra, R., Bosch, R. J., Cazaña, F. J. L., & Fernández-Martínez, A. B. (2019). Apoptosis and cell proliferation in proximal tubular cells exposed to apoptotic bodies. Novel pathophysiological implications in cisplatin-induced renal injury. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease.
• Fernández‐Bedmar, Z., Anter, J., & Alonso Moraga, A. (2018). Anti/genotoxic, longevity inductive, cytotoxic, and clastogenic‐related bioactivities of tomato and lycopene. Environmental and molecular mutagenesis, 59(5), 427-437.
• Luparello, C., Asaro, D. M. L., Cruciata, I., Hassell-Hart, S., Sansook, S., Spencer, J., & Caradonna, F. (2019). Cytotoxic Activity of the Histone Deacetylase 3-Selective Inhibitor Pojamide on MDA-MB-231 Triple-Negative Breast Cancer Cells. International Journal of Molecular Sciences, 20(4), 804.
• Fernández‐Bedmar, Z., Anter, J., & Moraga, Á. A. (2018). Anti/genotoxic, longevity inductive, cytotoxic, and clastogenic‐related bioactivities of tomato andlycopene. Environmental and molecular mutagenesis.
• Martínez-Valdivieso, D., Font, R., Fernández-Bedmar, Z., Merinas-Amo, T., Gómez, P., Alonso-Moraga, Á., & Del Río-Celestino, M. (2017). Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Antigenotoxicity, Cytotoxicity and Apoptotic Effects.  Nutrients, 9(7), 755.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What is the detection principle of this kit?
It detects apoptosis by binding phosphatidylserine with FITC-labeled Annexin V.

Q2: Which instruments can be used?
It is validated for flow cytometry and fluorescence microscopy.

Q3: Can this kit distinguish between apoptosis and necrosis?
Yes, when used with PI or 7-AAD, it differentiates viable, apoptotic, and necrotic cells.