SRB Cytotoxicity Assay Kit

Sensitive and reproducible colorimetric assay for cytotoxicity and cell viability.

The SRB Cytotoxicity Assay Kit provides a sensitive, reproducible, and easy-to-use method for measuring cell toxicity, viability, and proliferation. Based on the binding of the dye Sulforhodamine B (SRB) to cellular proteins, the assay delivers highly consistent colorimetric results.

The extracted dye, proportional to cell mass and protein content, is quantified at OD 540 nm with reference at 690 nm, providing excellent linearity, high signal-to-noise ratio, and stable end-point measurement. This makes the SRB Cytotoxicity Assay Kit a reliable alternative for both routine cytotoxicity testing and high-throughput screening.

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Price: 96.43 

SKU: CA050 Categories: ,

Detailed information:

Advantages & Features
  • Sensitive detection: quantifies subtle changes in cell mass.

  • Easy-to-use: straightforward workflow with minimal steps.

  • Fast results: avoids time-sensitive measurements.

  • Reproducible performance: consistent batch-to-batch accuracy.

  • Stable end-point: ensures reliable data storage and analysis.

Specifications
Includes

– 0.4 g SRB Dye
– 100 mL Fixative Reagent
– 100 mL Dye Wash Solution (10x)
– 200 mL SRB Solubization Buffer

Download documentation
Datasheet
MSDS
Applications
  • Cytotoxicity detection.

  • Cell viability assays.

  • Cell proliferation studies.

  • High-throughput screening.

Tables & Figures

Quality Control

Each batch is tested for consistency in SRB binding and reproducibility in cytotoxicity detection, ensuring linearity and low background.

Advice
  • Handle reagents with care; some are carcinogenic or corrosive.

  • Work in a fume hood with protective equipment (gloves, mask, goggles).

  • Protect the product from light.

Storage, Shipping & Guarantee
  • Shipped in: Ambient Temperature.
  • Storage: Room Temperature.
  • Recommendations: Protect the product from light.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review
FAQs

Q1: What wavelength is used for SRB detection?
OD is measured at 540 nm with a reference at 690 nm.

Q2: Can this kit be used for high-throughput applications?
Yes, it is validated for high-throughput cytotoxicity and proliferation assays.

Q3: Is the end-point stable?
Yes, SRB staining provides a stable end-point, allowing delayed analysis.

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