TBARS Assay Kit

– 4 x 0.53 g Thiobarbituric Acid (TBA)
– 40 mL Diluent 1 (contain Acetic Acid)
– 20 mL Diluent 2 (contain NaOH)
– 10 mL SDS Solution
– 250 μL MDA Standard (10mM)
– 20 mL H2O2 solution
– 1 unit 96-Well Solid Black plate
– 1 unit 96-Well Solid Clear plate
– 2 Adhesive strips

• Quantitative determination of lipid peroxides (TBARS).

• Evaluation of drug effects on lipid peroxidation.

• Oxidative stress and redox biology studies.

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• Protect reagents from light.

• Handle samples consistently for reproducible results.

• Shipment: Gel Pack.
• Storage: 4 °C upon receipt.

• Lagal, D. J., Ortiz-Alcántara, Á., Pedrajas, J. R., McDonagh, B., Bárcena, J. A., Requejo-Aguilar, R., & Padilla, C. A. (2024). Loss of peroxiredoxin 6 (PRDX6) alters lipid composition and distribution resulting in increased sensitivity to ferroptosis. Biochemical Journal, BCJ20240445.
• Martínez Cid, P. (2024). Innovación en modelos in vitro: Resistencia a la ferroptosis en células de cáncer de ovario epitelial.
• López Calvo, I. (2023). Estudio del papel de la ferroptosis en la resistencia al tratamiento quimioterapéutico en Cáncer de Ovario.
• Balart-García, P., Aristide, L., Bradford, T., Beasley-Hall, P., Polak, S., Ribera, I., … & Fernandez, R. (2022). Genomic exaptation and convergent evolution paved the way to independent subterranean colonization across beetle lineages.
• Alarcón-Veleiro, C., Mato-Basalo, R., Lucio-Gallego, S., Vidal-Pampín, A., Quindós-Varela, M., Al-Qatarneh, T., … & Fafián-Labora, J. (2023). Study of Ferroptosis Transmission by Small Extracellular Vesicles in Epithelial Ovarian Cancer Cells. Antioxidants, 12(1), 183.
• Lagal, D. J., López-Grueso, M. J., Pedrajas, J. R., Leto, T. L., Bárcena, J. A., Requejo-Aguilar, R., & Padilla, C. A. (2023). Loss of PRDX6 Aborts Proliferative and Migratory Signaling in Hepatocarcinoma Cell Lines. Antioxidants, 12(6), 1153.
• García-Sanmartín, J., Bobadilla, M., Mirpuri, E., Grifoll, V., Pérez-Clavijo, M., & Martínez, A. (2022). Agaricus Mushroom-Enriched Diets Modulate the Microbiota-Gut-Brain Axis and Reduce Brain Oxidative Stress in Mice. Antioxidants, 11(4), 695.
• Luque-Tévar, M., Perez-Sanchez, C., Patiño-Trives, A. M., Barbarroja, N., Arias de la Rosa, I., Abalos-Aguilera, M., … & Lopez-Pedrera, C. (2021). Integrative clinical, molecular, and computational analysis identify novel biomarkers and differential profiles of anti-TNF response in rheumatoid arthritis. Frontiers in immunology, 12, 592.
• Bobadilla, Miriam, et al. “A Grape Juice Supplemented with Natural Grape Extracts Is Well Accepted by Consumers and Reduces Brain Oxidative Stress.” Antioxidants 10.5 (2021): 677.
• Bobadilla, M., García-Sanmartín, J., & Martínez, A. (2021). Natural Food Supplements Reduce Oxidative Stress in Primary Neurons and in the Mouse Brain, Suggesting Applications in the Prevention of Neurodegenerative Diseases. Antioxidants, 10(1), 46.
• Fafián-Labora, J. A., Rodríguez-Navarro, J. A., & O’Loghlen, A. (2020). Small Extracellular Vesicles Have GST Activity and Ameliorate Senescence-Related Tissue Damage. Cell Metabolism.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

TBARS assay kit, Lipid peroxidation assay kit, Malondialdehyde assay kit, MDA detection assay, Thiobarbituric acid reactive substances assay

Also known as:

• Spanish: Kit de ensayo TBARS, Ensayo de peroxidación lipídica, Detección de malondialdehído
• French: Kit d’analyse TBARS, Test de peroxydation lipidique, Détection du malondialdéhyde
• German: TBARS-Testkit, Lipidperoxidations-Assay, Nachweis von Malondialdehyd
• Italian: Kit saggio TBARS, Saggio di perossidazione lipidica, Rilevazione malondialdeide

Q: What is the detection principle of the TBARS kit?
A: Reaction of MDA with TBA forms a chromogen measurable at 532 nm.

Q: Can this assay be used with serum or plasma?
A: Yes, validated for plasma, serum, urine, and tissue homogenates.

Q: Is the kit suitable for fluorometric detection?
A: Yes, compatible with both colorimetric and fluorometric methods.

Q: Why are EDTA and propyl gallate included in the extraction buffer?
A: They prevent artificial oxidation during sample preparation, ensuring accurate measurement of lipid peroxidation.

Q: Can samples be stored before performing the TBARS assay?
A: Yes, samples can be stored at −70 °C, but immediate analysis is recommended to avoid additional oxidation.

Q: How should meat or fish samples be prepared for lipid oxidation analysis?
A: Samples should be homogenized in 10% TCA containing EDTA and propyl gallate, followed by centrifugation to obtain a clear supernatant for analysis.