XTT Cell Proliferation Assay Kit
Sensitive, accurate, and fast XTT cell proliferation assay for colorimetric detection of cell viability and growth.
The XTT Cell Proliferation Assay Kit is a fast, sensitive, and reliable colorimetric method to quantify cell viability and proliferation. Based on the reduction of the yellow tetrazolium salt XTT to an orange formazan dye by dehydrogenase enzymes in metabolically active cells, this assay provides a direct correlation between dye intensity and viable cell number.
The XTT cell proliferation assay requires no solubilization step, making it time-saving and ideal for high-throughput screening. Its accuracy and reproducibility make it suitable for drug testing, toxicology, and cell biology studies. Formazan dye solubility in aqueous solution allows easy spectrophotometric measurement at 450 nm.
Have questions? Contact us
Price: 102.50 € – 194.60 €
Detailed information:
Advantages & Features
- Accurate: proportional to the number of viable cells.
- Sensitive: detects even low cell concentrations.
- Fast protocol: results in 2–5 hours.
- No solubilization step: reduces hands-on time.
- Safe: non-radioactive method.
- High-throughput optimized: compatible with 96-well/384-well plates.
Specifications
Includes
Includes for 1,000 assays:
– 2 x 25 mL XTT Cell Proliferation Assay Kit Reagent
– 1 mL Activation Reagent
Download documentation
Datasheet MSDSApplications
-
Cell proliferation and viability quantification.
-
Drug screening and cytotoxicity testing.
-
Toxicology assays with chemicals and nutrients.
-
Environmental compound effect analysis.
-
High-throughput screening in microplates.
Tables & Figures
Quality Control
-
Lot-to-lot reproducibility ensured.
-
Tested for sensitivity and linearity in cell-based assays.
Advice
-
Protect reagents from light.
-
Use freshly prepared activation reagent.
-
Include positive and negative controls for accuracy.
Storage, Shipping & Guarantee
- Shipped at: Gel Pack.
- Storage: -20 °C.
- Recommendations: Protect XTT solution from light.
Citations
- Carletti, A., Pes, K., Tarasco, M., Rosa, J. T., Poudel, S., Pereira, H., … & Gavaia, P. Immunomodulatory inhibition of osteoclastogenesis by a marine microalgal ethanol fraction targeting T-cells, antigen presentation, and macrophage fate. Frontiers in Immunology, 16, 1655321.
- Usala, E., Gonzalez, Z., Campillo, N., Baena, J., Ferrari, B., Rodríguez, A., & Espinosa, E. (2025). Impact of Steam Sterilization on the Rheological Characteristics, Printability, and Bioactivity of Nanocellulose/Alginate Hydrogels for 3D Bioprinting. Carbohydrate Polymer Technologies and Applications, 100970.
- Carletti, A., Pes, K., Tarasco, M., Rosa, J. T., Poudel, S., Pereira, H., … & Gavaia, P. J. (2025). Harnessing the immune system to treat bone loss: The immunomodulatory and osteoprotective effects of the microalga Skeletonema costatum. bioRxiv, 2025-02.
- Tavares Pereira, M., Kazemian, A., Rehrauer, H., & Kowalewski, M. P. (2022). Transcriptomic profiling of canine decidualization and effects of antigestagens on decidualized dog uterine stromal cells. Scientific Reports, 12(1), 21890.
- Kuczwara, V., Schuler, G., Pfarrer, C., Tiedje, L., Kazemian, A., Pereira, M. T., … & Klisch, K. (2023). Ultrastructural and Immunohistochemical Characterization of Maternal Myofibroblasts in the Bovine Placenta around Parturition. Veterinary Sciences, 10(1), 44.
- Hervás-Corpión, I., Navarro-Calvo, J., Martín-Climent, P., Iriarte-Gahete, M., Geribaldi-Doldán, N., Castro, C., & Valor, L. M. (2023). Defining a Correlative Transcriptional Signature Associated with Bulk Histone H3 Acetylation Levels in Adult Glioblastomas. Cells, 12(3), 374.
- Zaballos, M. A., Acuña-Ruiz, A., Morante, M., Riesco-Eizaguirre, G., Crespo, P., & Santisteban, P. (2022). Inhibiting ERK dimerization ameliorates BRAF-driven anaplastic thyroid cancer. Cellular and Molecular Life Sciences, 79(9), 1-19.
- Ramírez-Moya, J., Wert-Lamas, L., Acuña-Ruíz, A., Fletcher, A., Wert-Carvajal, C., McCabe, C. J., … & Riesco-Eizaguirre, G. (2022). Identification of an interactome network between lncRNAs and miRNAs in thyroid cancer reveals SPTY2D1-AS1 as a new tumor suppressor. Scientific reports, 12(1), 1-13.
- Ramírez-Moya, Julia, et al. “An ADAR1-dependent RNA editing event in the cyclin-dependent kinase CDK13 promotes thyroid cancer hallmarks.” Molecular Cancer 20.1 (2021): 1-20.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q: What is the detection principle of the XTT assay?
A: Reduction of XTT to a soluble formazan dye by viable cells.
Q: How long does the assay take?
A: Results in 2–5 hours.
Q: Does it require solubilization of the dye?
A: No, formazan is water-soluble.
11 reviews for XTT Cell Proliferation Assay Kit
You may also like…
-
Resazurin Cell Viability Assay
Price: 102.10 € CA035-SAdd to basket -
LDH Cytotoxicity Assay Kit
Price: 210.10 € – 559.10 € CA0020Select options This product has multiple variants. The options may be chosen on the product page -
ROS Detection Assay Kit
Price: 245.50 € CA093Add to basket -
XTT Cell Proliferation Assay Kit – 500 assays
Price: 102.50 € CA031-SAdd to basket
patricia –
We use this XTT assay routinely to monitor cell viability in 96-well plates. The protocol is straightforward and the soluble formazan makes the workflow much easier compared to assays that require an extra solubilization step. Absorbance readings at 450 nm are clear and reproducible.
Lucas Fernández –
We tested the XTT kit for cytotoxicity screening and found it very practical for high-throughput formats. The absence of a solubilization step simplifies plate handling, especially when processing multiple samples in parallel.
Priya Natarajan –
The XTT assay performs as expected for measuring metabolically active cells. It is particularly useful when working with multi-well formats, as the workflow is simple and does not involve additional processing steps.
Jonathan Miller –
This XTT kit is reliable for standard viability measurements. The soluble formazan product is a clear advantage in terms of workflow simplicity and reproducibility between runs.
Noor Al-Hassan –
A practical assay for cell viability and proliferation studies. The protocol is uncomplicated, and the results are easy to interpret using a conventional spectrophotometer.
Amina El-Sayed –
The kit works well for standard proliferation measurements based on metabolic activity. I appreciate that the color develops directly in the medium, which saves time during routine experiments. Instructions are clear and easy to follow.
Martin Vogel –
Good option for routine cell proliferation assays. The reagents are easy to handle, and the assay principle is well documented, which makes it suitable for training new lab members.
Mei-Ling Chen –
This assay fits well into our regular cell viability workflow. The colorimetric readout is easy to quantify with a standard plate reader, and results are consistent when cell density is properly optimized.
Robert Hastings –
We chose this XTT assay because of its simple protocol and water-soluble formazan. It integrates smoothly into our existing cell-based assays without requiring changes to our equipment or readout settings.
Sofia Marino –
The kit is convenient for proliferation studies where fast turnaround is needed. From reagent preparation to absorbance measurement, the process is efficient and well suited for routine laboratory use.
Tomasz Zieliński –
We use this assay mainly for comparative viability studies. The color development is stable enough for flexible reading times, which helps when running several plates in the same experiment.