Recombinant Human EGF (52 aa)
High-purity recombinant human EGF (52 aa), produced in E. coli, carrier-free, animal-origin free and low endotoxin. Highly active in epithelial, neural and organoid models.
Recombinant Human EGF (52 aa) is a high-purity, carrier-free growth factor produced in E. coli and designed for robust, reproducible activity across epithelial, neural, mesenchymal and organoid culture systems. Corresponding to the mature 52-amino-acid human EGF domain, this recombinant form promotes the expansion, proliferation and differentiation of ESC/iPSC-derived epithelial lineages, primary cells and a broad range of organoid models, including intestine, stomach, liver, pancreas, brain and tumor organoids.
Its chemically defined, animal-origin–free composition and stringent control of endotoxin levels ensure consistent, reliable performance in sensitive stem-cell and organoid workflows. Delivered as a stable lyophilized powder, the protein offers easy handling, high recovery and minimal batch-to-batch variation—ideal for advanced media formulations and high-performance culture systems.
A GMP-grade manufacturing option is available for regulated environments and therapeutic workflows. Contact us.
Price: 72.00 €
Detailed information:
Advantages & Features
- High-purity recombinant EGF (52 aa): ideal for defined stem-cell and organoid media
- Strong and consistent bioactivity: validated in SRE-luc reporter assays (EC50 ~70 pg/mL).
- Animal-origin free & carrier-free: fully chemically defined formulation.
- Batch-to-batch consistency ensured through controlled manufacturing and analytical release testing.
- Low endotoxin: <0.05 EU/µg, suitable for sensitive epithelial and organoid systems.
- Stable lyophilized format: easy handling, long-term storage and high lot-to-lot reproducibility.
GMP-grade version is available with extended release QC testing and full regulatory documentation. Contact us.
Specifications
- Expression system: E. coli recombinant
- Sequence: Human EGF (52 aa), corresponding to residues 971–1023 UniProt P01133
- Molecular weight: ~6.3 kDa (monomer)
- Purity: >98% (SDS-PAGE)
- Form: Lyophilized powder
- Bioactivity: SRE-luc reporter assay (EC50 ~70 pg/mL)
- Endotoxin: <0.05 EU/µg protein
- Additional testing: Mass spectrometry, recovery >95%
- Recombinant core active region (52 aa): mature 52 aa EGF
A GMP version is available for therapeutic or regulated manufacturing workflows. Contact us.
Includes
1 vial with 100μg of EGF
Download documentation
Datasheet MSDSApplications
Recombinant human EGF (52 aa) is widely used for organoid culture and stem-cell–derived epithelial differentiation workflows.
- Organoid culture systems: intestine, stomach, liver, pancreas, brain and tumor organoids
- Epithelial, neural and mesenchymal stem-cell differentiation.
- Expansion and differentiation of ESC/iPSC-derived epithelial and neural lineages.
- Proliferation of fibroblasts, chondrocytes and primary cells.
- Media supplementation in organoid niche factor cocktails (EGF, R-Spondin, Noggin/Gremlin, Wnt3a, FGF-10).
Additional recombinant growth factors are available to support complementary or alternative cell-culture applications.
A GMP-grade variant of EGF is available for cell therapy, regenerative medicine and advanced bioprocessing. Contact us.
Tables & Figures
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Quality Control
- Purity ≥98% (SDS-PAGE)
- Identity confirmed by mass spectrometry
- Bioactivity validated by SRE-Luc reporter assay
- Endotoxin <0.05 EU/µg protein
- Recovery >95% after reconstitution
- Sterility confirmed (no microbial growth detected)
- Mycoplasma negative
GMP-grade batches include extended QC testing (sterility, mycoplasma, host-cell impurities). Contact us.
Advice
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Reconstitute in 10 mM HCl at >50 µg/mL for optimal stability.
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Prepare aliquots and store frozen to avoid multiple freeze–thaw cycles.
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Use carrier-free conditions to maintain consistent activity.
Storage, Shipping & Guarantee
Shipped at room temperature.
Store at –20 °C for general use. Avoid repeated freeze–thaw cycles. Once reconstituted, aliquot and store at –80 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
GMP-grade production can be provided on request. Full regulatory documentation is available for GMP-manufactured batches.. Please Contact us for specifications, documentation and batch availability.
Customers Review
FAQs
Why is EGF widely used in epithelial and organoid-based models?
Recombinant human EGF is a potent activator of EGFR signaling, inducing rapid and robust mitogenic responses in epithelial cells. This makes EGF a core component of organoid culture systems and epithelial differentiation workflows, including models increasingly used in translational and preclinical research.
How does EGF contribute to reproducible organoid and differentiation systems?
EGF supports consistent epithelial proliferation and tissue organization through well-characterized MAPK and PI3K/AKT signaling pathways. Its chemically defined, animal-origin–free formulation facilitates standardization of media formulations, a key requirement in advanced development and comparability studies.
Is recombinant EGF suitable for defined and serum-free workflows?
Yes. Carrier-free recombinant human EGF is well suited for chemically defined and serum-free media, where precise control of growth-factor signaling is required. These features are particularly relevant for media development and translational research in regenerative and therapeutic applications.
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