Recombinant Human EGF (52 aa)

• Expression system: E. coli recombinant
• Sequence: Human EGF (52 aa), corresponding to residues 971–1023 UniProt P01133
• Molecular weight: ~6.3 kDa (monomer)
• Purity: >98% (SDS-PAGE)
• Form: Lyophilized powder
• Bioactivity: SRE-luc reporter assay (EC50 ~70 pg/mL)
• Endotoxin: <0.05 EU/µg protein
• Additional testing: Mass spectrometry, recovery >95%
• Recombinant core active region (52 aa): mature 52 aa EGF

A GMP version is available for therapeutic or regulated manufacturing workflows. Contact us.

1 vial with 100μg of EGF

1 vial with 1mL of 10 mM HCl (solution for reconstitution)

Recombinant human EGF (52 aa) is widely used for organoid culture and stem-cell–derived epithelial differentiation workflows.

• Organoid culture systems: intestine, stomach, liver, pancreas, brain and tumor organoids
• Epithelial, neural and mesenchymal stem-cell differentiation.
• Expansion and differentiation of ESC/iPSC-derived epithelial and neural lineages.
• Proliferation of fibroblasts, chondrocytes and primary cells.
• Media supplementation in organoid niche factor cocktails (EGF, R-Spondin, Noggin/Gremlin, Wnt3a, FGF-10).

Additional recombinant growth factors are available to support complementary or alternative cell-culture applications.

We also offer a GMP-grade manufacturing option for regulated environments and therapeutic workflows. Contact us.

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• Purity ≥98% (SDS-PAGE)
• Identity confirmed by mass spectrometry
• Bioactivity validated by SRE-Luc reporter assay
• Endotoxin <0.05 EU/µg protein
• Recovery >95% after reconstitution
• Sterility confirmed (no microbial growth detected)
• Mycoplasma negative

GMP-grade batches include extended QC testing (sterility, mycoplasma, host-cell impurities). Contact us.

• Reconstitute in 10 mM HCl at >50 µg/mL for optimal stability.

• Prepare aliquots and store frozen to avoid multiple freeze–thaw cycles.

• Use carrier-free conditions to maintain consistent activity.

Shipped at room temperature.
Store at –20 °C for general use. Avoid repeated freeze–thaw cycles. Once reconstituted, aliquot and store at –80 °C.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

GMP-grade production can be provided on request. Please Contact us for specifications, documentation and batch availability.

EGF protein, Epidermal growth factor, Recombinant EGF, Human EGF protein, EGF growth factor

Also known as:

• Spanish: EGF recombinante, Factor de crecimiento epidérmico, EGF humano
• French: EGF recombinant, Facteur de croissance épidermique, EGF humain
• German: Rekombinantes EGF, Epidermaler Wachstumsfaktor, EGF human
• Italian: EGF ricombinante, Fattore di crescita epidermico, EGF umano

Why is EGF widely used in epithelial and organoid-based models?

Recombinant human EGF is a potent activator of EGFR signaling, inducing rapid and robust mitogenic responses in epithelial cells. This makes EGF a core component of organoid culture systems and epithelial differentiation workflows, including models increasingly used in translational and preclinical research.

How does EGF contribute to reproducible organoid and differentiation systems?

EGF supports consistent epithelial proliferation and tissue organization through well-characterized MAPK and PI3K/AKT signaling pathways. Its chemically defined, animal-origin–free formulation facilitates standardization of media formulations, a key requirement in advanced development and comparability studies.

Is recombinant EGF suitable for defined and serum-free workflows?

Yes. Carrier-free recombinant human EGF is well suited for chemically defined and serum-free media, where precise control of growth-factor signaling is required. These features are particularly relevant for media development and translational research in regenerative and therapeutic applications.