Recombinant Human FGF-2 (145 aa)
High-purity recombinant human FGF-2 produced in E. coli. animal-origin free, endotoxin-controlled and validated for robust activity in stem-cell applications.
Recombinant Human FGF-2 (145 aa) is a high-purity, carrier-free growth factor produced in E. coli and designed to deliver robust, reproducible activity in advanced stem–cell research workflows. Corresponding to the 145-aa core region of human fibroblast growth factor 2, this form actively supports the expansion and maintenance of induced pluripotent stem cells (iPSCs), embryonic stem cells (ESCs), mesenchymal stem cells (MSCs), and a broad range of proliferation and differentiation models.
Its animal-origin–free composition and stringent control of endotoxin levels ensure consistent biological performance in sensitive cell-culture systems. Supplied as a stabilized lyophilized powder, the protein enables easy reconstitution, high recovery, and lot-to-lot consistency, making it ideal for demanding research workflows.
A GMP-grade version of the Recombinant Human FGF-2 (145 aa) suitable for therapeutic manufacturing is also available upon request. Contact us.
Price: 180.00 €
Detailed information:
Advantages & Features
- High Purity & Bioactivity: ≥98% purity (SDS-PAGE) with reproducible activity in validated SRE luciferase reporter assays.
- Animal-Origin Free: Fully AOF and carrier-free formulation for sensitive stem-cell applications.
- Reliable Stem-Cell Support: Optimized for iPSC, ESC and MSC maintenance, proliferation and differentiation models.
- Batch-to-batch consistency ensured through controlled manufacturing and analytical release testing.
- Low Endotoxin: <0.05 EU/µg suitable for high-performance cell-culture workflows.
- Stable Lyophilized Format: Vacuum-dried for maximum stability and easy reconstitution.
GMP-grade version is available with extended release testing and documentation for clinical and biomanufacturing use. Contact us.
Specifications
- Expression system: E. coli recombinant.
- Sequence: Human FGF-2 (145 aa; residues 144–288, UniProt P09038).
- Molecular weight: 17 kDa (monomer).
- Purity: >98% (SDS-PAGE).
- Form: Lyophilized powder.
- Bioactivity: SRE-Luc assay in HEK293T cells (EC50 ~0.24 ng/mL).
- Endotoxin: <0.05 EU/µg protein.
- Additional testing: Mass spectrometry, recovery >95%
- Recombinant Core Active Region 145 aa: biologically active form, ensuring robust FGFR signaling.
GMP version available for use in regulated therapeutic manufacturing workflows. Contact us.
Includes
1 vial with 100μg of FGF-2
Download documentation
Datasheet MSDSApplications
The Recombinant Human FGF-2 (145 aa) is widely used for stem-cell expansion and maintenance, as well as in angiogenesis and proliferation-driven cell-culture workflows.
- iPSC and ESC maintenance.
- MSC proliferation and differentiation.
- Stem-cell media supplementation.
- FGFR1–4 signaling studies.
- Cell-proliferation and survival assays.
- Regenerative biology, wound-healing and angiogenesis research.
Additional recombinant growth factors are available to support complementary or alternative cell-culture applications.
GMP-grade variant is also offered for applications in cell therapy, regenerative medicine and advanced therapeutic manufacturing. Contact us.
Tables & Figures
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Quality Control
- Purity ≥98% (SDS-PAGE)
- Identity confirmed by mass spectrometry
- Bioactivity validated by SRE-Luc reporter assay
- Endotoxin <0.05 EU/µg protein
- Recovery >95% after reconstitution
- Sterility confirmed (no microbial growth detected)
- Mycoplasma negative
GMP-grade option includes additional QC testing such as sterility, mycoplasma and host-cell impurity controls. Contact us.
Advice
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Reconstitute in sterile water at >50 µg/mL for optimal stability.
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Prepare aliquots and store frozen to avoid multiple freeze–thaw cycles.
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Use carrier-free conditions to maintain consistent activity.
Storage, Shipping & Guarantee
Shipped at room temperature.
Store at –20 °C for general use. Avoid repeated freeze–thaw cycles. Once reconstituted, aliquot and store at –80 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
GMP-grade production can be provided on request. Full regulatory documentation is available for GMP-manufactured batches.. Please Contact us for specifications, documentation and batch availability.
Customers Review
FAQs
Why is FGF-2 a key growth factor in stem-cell expansion workflows?
Recombinant human FGF-2 plays a central role in the expansion and maintenance of pluripotent and mesenchymal stem cells, supporting controlled proliferation while preserving key cellular characteristics. These properties make FGF-2 widely used not only in basic research, but also in process development and preclinical workflows related to cell therapy and regenerative medicine.
Why is FGF-2 a key growth factor in stem-cell expansion workflows?
Recombinant human FGF-2 plays a central role in the expansion and maintenance of pluripotent and mesenchymal stem cells, supporting controlled proliferation while preserving key cellular characteristics. These properties make FGF-2 widely used not only in basic research, but also in process development and preclinical workflows related to cell therapy and regenerative medicine.
In which advanced applications is FGF-2 commonly applied?
FGF-2 is widely used in stem-cell expansion, angiogenesis-related models and proliferation-driven culture systems. It is frequently included in development-stage workflows supporting regenerative medicine, tissue engineering and cell-based therapeutic research.
