Recombinant Human IGF-1 (70 aa)
High-purity recombinant human IGF-1 (70 aa), produced in E. coli, carrier-free, AOF and low endotoxin. Highly active in pluripotent and defined cell-culture systems. Stable lyophilized format.
Recombinant Human IGF-1 (70 aa) is a high-purity, carrier-free growth factor produced in E. coli. It is engineered to deliver potent and reproducible biological activity across pluripotent stem-cell systems and defined culture workflows. The protein corresponds to the mature 70-amino-acid human IGF-1 sequence.
This recombinant IGF-1 supports proliferation, survival and metabolic regulation in iPSC, ESC, MSC and primary cell models. It is widely used as an insulin substitute in chemically defined, serum-free and xeno-free media formulations.
Its chemically defined, animal-origin–free composition is combined with stringent endotoxin and analytical control. This ensures exceptional lot-to-lot reproducibility and makes the product suitable for sensitive culture systems and advanced bioprocess development. Supplied as a stable lyophilized powder, IGF-1 enables efficient reconstitution, high recovery and robust performance in demanding workflows.
A GMP-grade manufacturing option is available for regulated environments and therapeutic workflows. Contact us.
Price: 72.00 €
Detailed information:
Advantages & Features
- High-purity recombinant IGF-1 (70 aa): ideal for pluripotent stem-cell and defined media systems.
- Strong biological activity: validated using SRE-luc reporter assays (EC50 ≈ 3.5 ng/mL).
- Animal-origin free & carrier-free formulation: fully chemically defined.
- Batch-to-batch reproducibility: controlled production and analytical release testing.
- Low endotoxin: <0.05 EU/µg for sensitive stem-cell and primary-cell workflows.
- Stable lyophilized format: easy handling and long-term storage.
GMP-grade version is available with extended release QC testing and full regulatory documentation. Contact us.
Specifications
- Expression system: E. coli recombinant
- Sequence: Human IGF-1 (70 aa), UniProt P05019
- Molecular weight: ~7.6–8 kDa (monomer)
- Structure: Single-chain growth factor containing intramolecular disulfide bonds
- Purity: ≥98% (SDS-PAGE)
- Identity: Confirmed by mass spectrometry
- Form: Lyophilized powder
- Bioactivity: SRE-luc assay in MCF-7 cells (EC50 ≈ 3.5 ng/mL)
- Endotoxin: <0.05 EU/µg protein
- Additional testing: Mass spectrometry, recovery >95%
- Recombinant core active region: mature 70-aa IGF-1 sequence.
A GMP version is available for therapeutic or regulated manufacturing workflows. Contact us.
Includes
1 vial with 100 μg of IGF-1
Download documentation
Datasheet MSDSApplications
Recombinant human IGF-1 (70 aa) is widely used for pluripotent stem-cell culture and as an insulin replacement in chemically defined media.
- Maintenance of pluripotency in human iPSC and ESC cultures.
- Replacement of insulin in chemically defined media.
- Proliferation and survival of MSCs and primary cells.
- Development of serum-free and xeno-free media formulations.
- Defined media compositions for epithelial and mesenchymal systems.
Additional recombinant growth factors are available to support complementary or alternative cell-culture applications.
A GMP-grade variant of IGF-1 is available for cell therapy, regenerative medicine and advanced bioprocessing. Contact us.
Tables & Figures
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Quality Control
- Purity ≥98% (SDS-PAGE)
- Identity confirmed by mass spectrometry
- Bioactivity validated by SRE-Luc reporter assay
- Endotoxin <0.05 EU/µg protein
- Recovery >95% after reconstitution
- Sterility confirmed (no microbial growth detected)
- Mycoplasma negative
GMP-grade batches include extended QC testing (sterility, mycoplasma, host-cell impurities). Contact us.
Advice
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Reconstitute in sterile water at >50 µg/mL for optimal stability.
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Prepare single-use aliquots and store at –80 °C. Avoid repeated freeze–thaw cycles.
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Use carrier-free conditions to maintain consistent activity.
Storage, Shipping & Guarantee
Shipped at room temperature.
Store at –20 °C for general use. Avoid repeated freeze–thaw cycles. Once reconstituted, aliquot and store at –80 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
GMP-grade production can be provided on request. Full regulatory documentation is available for GMP-manufactured batches.. Please Contact us for specifications, documentation and batch availability.
Customers Review
FAQs
What role does IGF-1 play in advanced cell-culture workflows?
Recombinant human IGF-1 activates IGF-1R signaling pathways that regulate cell survival, metabolism and controlled proliferation. This makes IGF-1 highly relevant in defined culture systems, survival-driven assays and development-stage workflows supporting translational and preclinical research.
Why is IGF-1 commonly used as an insulin replacement in defined media?
IGF-1 is frequently used as an insulin substitute in chemically defined and serum-free media, where it supports metabolic activity and cell viability without introducing animal-derived components. This approach is widely adopted during media optimization and process development for advanced applications.
Which applications benefit most from recombinant IGF-1?
IGF-1 is broadly applied in pluripotent stem-cell culture, primary cell survival studies and defined media formulations. Its role in metabolic and survival signaling makes it particularly valuable in translational research and early-stage therapeutic development.
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