Recombinant Human PDGF-BB
High-purity recombinant human PDGF-BB (25 kDa dimer), produced in E. coli, animal-origin free, carrier-free and highly bioactive. Ideal for MSC expansion, neuronal differentiation and angiogenesis workflows. Stable lyophilized format.
Recombinant Human PDGF-BB is a high-purity, carrier-free dimeric growth factor produced in E. coli and designed to deliver strong, reproducible biological activity in mesenchymal, neuronal and angiogenesis-related culture systems. This recombinant protein corresponds to the biologically active PDGF-B homodimer, a disulfide-linked 25 kDa dimer involved in cellular proliferation, migration, survival and tissue-repair pathways.
Widely used in MSC expansion, neural stem-cell differentiation, osteogenic models and angiogenesis workflows, PDGF-BB is a key component of many defined and serum-free media formulations. Its chemically defined, animal-origin–free composition and stringent QC controls—including purity, endotoxin, sterility and mycoplasma—ensure consistent performance in sensitive culture systems.
Supplied as a stable lyophilized powder, the protein guarantees easy handling, efficient reconstitution, and robust lot-to-lot reproducibility.
A GMP-grade manufacturing option is available for regulated environments and therapeutic workflows. Contact us.
Price: 391.50 €
Detailed information:
Advantages & Features
- High-purity disulfide-linked PDGF-BB dimer: ~25 kDa active homodimer.
- Strong and reproducible activity: validated via NFAT-luciferase assays (EC50 ≈ 12.7 ng/mL).
- Animal-origin free & carrier-free: fully chemically defined formulation.
- Ideal for mesenchymal, neuronal and angiogenic systems: supports proliferation, differentiation and migration.
- Controlled batch-to-batch reproducibility: tight analytical release testing.
- Low endotoxin: <0.05 EU/µg suitable for sensitive culture systems
- Stable lyophilized format: long-term storage, consistent reconstitution.
GMP-grade version is available with extended release QC testing and full regulatory documentation. Contact us.
Specifications
- Expression system: E. coli recombinant.
- Sequence: Human PDGF-BB homodimer, UniProt P01127
- Molecular weight: ~25 kDa dimer (non-reducing), ~13 kDa monomer (reducing)
- Structure: Disulfide-linked PDGF-B/PDGF-B homodimer
- Purity: ≥98% (SDS-PAGE)
- Identity: Mass spectrometry
- Form: Lyophilized powder
- Bioactivity: NFAT-luc assay (EC50 ≈ 12.7 ng/mL)
- Endotoxin: <0.05 EU/µg protein
- Additional testing: Recovery >95%
- Recombinant active region: recombinant disulfide-linked PDGF-BB homodimer.
A GMP version is available for therapeutic or regulated manufacturing workflows. Contact us.
Includes
1 vial with 500 μg of PDGF-BB
Download documentation
Datasheet MSDSApplications
Recombinant human PDGF-BB is widely used for MSC expansion and angiogenesis-related cell-culture workflows.
- Proliferation and migration of mesenchymal cells (fibroblasts, MSCs, VSMCs).
- Neuronal differentiation and neural stem-cell expansion.
- iPSC/ESC and MSC culture workflows.
- Osteogenic models and bone-related differentiation.
- Angiogenesis, wound-healing and vascular biology studies.
- Supplementation of defined and serum-free mesenchymal/neural media.
Additional recombinant growth factors are available to support complementary or alternative cell-culture applications.
A GMP-grade variant of PDGF-BB is available for cell therapy, regenerative medicine and advanced bioprocessing. Contact us.
Tables & Figures
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Quality Control
- Purity ≥98% (SDS-PAGE)
- Identity confirmed by mass spectrometry
- Bioactivity validated by NFAT-luciferase reporter assay
- Endotoxin <0.05 EU/µg protein
- Recovery >95% after reconstitution
- Sterility confirmed (no microbial growth detected)
- Mycoplasma negative
GMP-grade batches include extended QC testing (sterility, mycoplasma, host-cell impurities). Contact us.
Advice
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Reconstitute in sterile water at >50 µg/mL for optimal stability.
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Prepare single-use aliquots and store at –80 °C. Avoid repeated freeze–thaw cycles.
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Use carrier-free conditions to maintain consistent activity.
Storage, Shipping & Guarantee
Shipped at room temperature.
Store at –20 °C for general use. Avoid repeated freeze–thaw cycles. Once reconstituted, aliquot and store at –80 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
GMP-grade production can be provided on request. Full regulatory documentation is available for GMP-manufactured batches. Please Contact us for specifications, documentation and batch availability.
Customers Review
FAQs
Which applications benefit most from recombinant IGF-1?
IGF-1 is broadly applied in pluripotent stem-cell culture, primary cell survival studies and defined media formulations. Its role in metabolic and survival signaling makes it particularly valuable in translational research and early-stage therapeutic development.
How is PDGF-BB applied in migration and angiogenesis-related studies?
PDGF-BB is widely used in migration assays, wound-healing models and angiogenesis-related workflows, where precise modulation of mesenchymal signaling is required. These applications are commonly explored during preclinical evaluation and translational research.
Is PDGF-BB suitable for defined and serum-free media development?
Yes. The carrier-free, animal-origin–free formulation of recombinant human PDGF-BB ensures reproducible performance in defined and serum-free systems, supporting media development, comparability studies and advanced research workflows.
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