CANFAST™ Transfection Reagent
High-efficiency, non-toxic DNA transfection reagent with fast protocol and reproducible results in adherent and suspension cells
CanFast™ Transfection Reagent from Canvax™ is a ready-to-use reagent developed for the efficient delivery of DNA or RNA into a wide range of eukaryotic cells. It allows a fast and simple transfection procedure with high reproducibility and minimal cytotoxicity.
This reagent is suitable for transient or stable transfection experiments in various cell lines. Its optimized formulation ensures reliable performance and consistent gene expression in molecular and cell biology research.
With proven performance across a broad range of mammalian cell types, CANFAST™ ensures cost-effective and reliable results for gene expression, co-transfection, and primary cell studies.
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Price: 51.80 €
Detailed information:
Advantages & Features
- High transfection efficiency in most mammalian cell lines.
- Non-toxic: cell viability consistently >90%.
- Serum-compatible: works in complete media.
- Time-saving protocol: successful transfections in 24 minutes.
- Broad applicability: adherent, suspension, stable and transient transfections.
- Cost-effective: requires minimal DNA and no media change.
Specifications
Includes
-1 mL CANFAST™ Transfection Reagent (1 µg/µL)
-10 µL GFP-Plasmid Transfection Control (1 µg/µL)
(1 mL = 250 – 500 transfections)
Download documentation
Datasheet MSDSBrochureFlyerApplications
- High-throughput DNA transfection.
- Stable & transient transfection assays.
- Co-transfection experiments.
- Primary cell transfection.
- Adherent and suspension cell lines.
- Gene expression & cellular analysis studies.
Tables & Figures
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Quality Control
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Transfection efficiency validated with GFP-expression plasmid in Jurkat cells (>50%).
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Lot-to-lot consistency ensured.
Advice
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Optimize DNA:reagent ratio for each cell type.
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Use serum-containing media for improved cell survival.
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Gently mix, do not vortex, to preserve reagent activity.
Storage, Shipping & Guarantee
- Shipped in: Gel pack
- Storage: 4 ºC.
Citations
- Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
- Gómez-Melero, S., García-Maceira, F. I., García-Maceira, T., Luna-Guerrero, V., Montero-Peñalvo, G., Caballero-Villarraso, J., … & Paz-Rojas, E. (2022). Development of a High-Throughput Calcium Mobilization Assay for CCR6 Receptor Coupled to Hydrolase Activity Readout. Biomedicines, 10(2), 422.
- Gómez-Melero, S., García-Maceira, F. I., García-Maceira, T., Luna-Guerrero, V., Montero-Peñalvo, G., Túnez-Fiñana, I., & Paz-Rojas, E. (2021). Amino Terminal Recognition by a CCR6 Chemokine Receptor Antibody Blocks CCL20 Signaling and IL-17 Expression via β-arrestin.
- Yago-Ibáñez, J., García-Pastor, C., Lucio-Cazaña, F. J., & Fernández-Martínez, A. B. (2020). Retinoic acid receptor-beta prevents cisplatin-induced proximal tubular cell death. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 165795.
- Pilná, H. (2019). Vliv interferon regulujícího faktoru 3 na imunitní odpověď proti viru vakcínie v atopickém organismu.
- Olmos, G., Martínez‐Miguel, P., Alcalde‐Estevez, E., Medrano, D., Sosa, P., Rodríguez‐Mañas, L., … & López‐Ongil, S. (2017). Hyperphosphatemia induces senescence in human endothelial cells by increasing endothelin‐1 production. Aging Cell.
- Martínez-Miguel, P., Valdivielso, J. M., Medrano-Andrés, D., Román-García, P., Cano-Peñalver, J. L., Rodríguez-Puyol, M., … & López-Ongil, S. (2014). The active form of vitamin D, calcitriol, induces a complex dual upregulation of endothelin and nitric oxide in cultured endothelial cells. American Journal of Physiology-Endocrinology and Metabolism, 307(12), E1085-E1096.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q: How many transfections can 1 mL provide?
A: Between 250–500, depending on cell type and format.
Q: Is it compatible with serum?
A: Yes, CANFAST™ works efficiently in complete media.
Q: Can I use it for co-transfection?
A: Yes, it supports co-delivery of multiple plasmids.
5 reviews for CANFAST™ Transfection Reagent
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Elena Verschueren –
Finding an affordable yet high-performing transfection reagent was simple on this site due to the clear and honest product information. The delivery was prompt, and the reagent worked perfectly for my CRISPR-Cas9 delivery experiments. It helped me overcome the problem of poor DNA uptake in primary cells, leading to a much higher success rate in my genomic edits. The ease of use and the fact that it works well in the presence of serum are huge advantages for our daily workflow. This was a great purchase and I will definitely be using it for all my future trials.
Nicolas Ghesquière –
I had a very positive experience ordering the CanFast™ Transfection Reagent, as the website is user-friendly and the shipping to Brussels was quite efficient. Everything was clearly described on the page, so I felt confident in choosing this reagent for my cell-based assays. This product solved my issue with inconsistent transfection rates in 24-well plates, providing a more uniform distribution of the transgene. The quality is top-notch and it has certainly improved our lab’s general productivity. I am giving it four stars instead of five only because I hope for more detailed optimization guides for rare cell types, but for standard lines, it is perfect.
Victor De Smet –
I am very pleased with the CanFast™ Transfection Reagent as it has significantly improved the reproducibility of my experiments. The purchase was easy to complete, and the fast shipping ensured that our project timeline stayed on track. This reagent solved the challenge of obtaining consistent results across different batches of cells, providing a very stable performance. It is a high-quality product that delivers exactly what it promises, making our research much more efficient. Overall, I am very impressed with both the product quality and the customer support.
Alice Dumont –
My experience with this purchase was excellent, as the product description was very clear and the checkout process was seamless. The reagent arrived well-packaged in dry ice to maintain its activity, which shows great attention to quality. This product effectively solved the issues I was having with low protein yield in my transient expression assays. The protocol is very simple and doesn’t require removing the medium, which saved me a lot of time in the lab. I highly recommend CanFast for anyone looking for a reliable and easy-to-use transfection tool
Johan Mertens –
I purchased the CanFast™ Transfection Reagent for my gene expression studies, and the ordering process was very straightforward and efficient. The website provided detailed technical protocols, and the delivery arrived in Brussels much faster than I had anticipated. This reagent genuinely helped me by providing high transfection efficiency even in difficult-to-transfect cell lines. It solved my previous problem with high cell toxicity, as my cells remained healthy and viable after the procedure. I am extremely satisfied with the results and the professional service.