BL21 (DE3) Competent Cells

Genotype: F¯ ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

– 10 µL BL21 (DE3) Competent Cells Control Plasmid (0.1 ng/µL)
– 10 x 200μL BL21 (DE3) Competent Cells
– Dry Ice

• Recombinant protein expression under T7 promoter.

• Production of toxic or unstable proteins.

• Routine cloning and expression studies.

• BL21(DE3) Competent Cells consistently yield > 1.0 × 10⁶ colony-forming units/µg competent cells control plasmid when transformed with non-saturating amounts of DNA (0.2ng/100 µL cells).
• Lot-to-lot reproducibility ensured.

• Store at –80 ºC upon receipt.

• Avoid repeated freeze-thaw cycles.

• Induce protein expression with IPTG for optimal results.

• Shipment with Dry Ice.
• Storage: Upon receipt of the kit, immediately store the components at -80 °C. Storage at -20 °C will result in a significant decrease in transformation efficiency. Please note that Competent Cells are very sensitive to cycles of freezing and thawing and should not be exposed to temperature variations.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q: What is the main use of BL21 (DE3) cells?
A: High-level recombinant protein expression using T7 promoter constructs.

Q: Why are they protease deficient?
A: To reduce degradation of recombinant proteins.

Q: What is their transformation efficiency?
A: ≥1 × 10⁶ CFU/µg DNA.