CVX5a™ Chemically Competent Cells

CVX5α™ Genotype:  F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ).

Includes for 40 rxn:
– 2,000 µL CVX5α™ (1 x 10⁷ CFU/µg)
– 10 µL pUC18 Transformation Control Plasmid (10 ng/µL)
– 50 mL SOC Medium
– Dry ice

• Routine cloning and subcloning into plasmid vectors.

• Blue/white screening of colonies.

• Gene cloning for sequencing or expression studies.

• Each lot of competent cells is tested to verify transformation efficiencies using 100 pg pUC18 supercoiled DNA and the recommended protocol.
• Under these conditions, transformation efficiency will be ≥ 1 x 10⁷ cfu/μg pUC18.
• Transformation efficiency is defined as the number of colony forming units (cfu) produced by transforming 1 μg of plasmid (3 kb) into a given volume of competent cells.

• Avoid multiple freeze-thaw cycles.

• Store immediately at –80 ºC upon receipt.

• Not suitable for cDNA library generation.

• Shipped in: Dry ice.
• Storage: Upon receipt, store cells immediately at -80 °C.
• Recommendations: Storage at -20 °C will result in a significant decrease in transformation efficiency. Please note that competent cells are very sensitive to cycles of freezing and thawing and should not be exposed to temperature variations

• Alvarez, J. B., Castellano, L., & Guzmán, C. (2025). Molecular characterization of the variability of the GLU-Hch1 gene in Hordeum chilense. Applied Food Research, 100784.
• Balart-García, P., Aristide, L., Bradford, T., Beasley-Hall, P., Polak, S., Ribera, I., … & Fernandez, R. (2022). Genomic exaptation and convergent evolution paved the way to independent subterranean colonization across beetle lineages.
• Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
• Alvarez, J. B., Castellano, L., Huertas-García, A. B., & Guzmán, C. (2021). Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion. Plant Science, 110843.
• Cabrera, A., Castellano, L., Recio, R., & Alvarez, J. B. (2019). Chromosomal location and molecular characterization of three grain hardness genes in Agropyron cristatum. Euphytica, 215(10), 165.
• Alvarez, J. B., Castellano, L., Recio, R., & Cabrera, A. (2019). Wx Gene in Hordeum chilense: Chromosomal Location and Characterisation of the Allelic Variation in the Two Main Ecotypes of the Species. Agronomy, 9(5), 261.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Chemically competent E. coli cells, Competent cells for transformation, E. coli cloning competent cells, High efficiency competent cells, DNA transformation cells

Also known as:

• Spanish: Células competentes químicamente, Células E. coli para transformación, Células competentes de clonación
• French: Cellules compétentes chimiquement, Cellules E. coli pour transformation, Cellules compétentes de clonage
• German: Chemisch kompetente Zellen, E. coli Transformationszellen, Klonierungs-kompetente Zellen
• Italian: Cellule competenti chimicamente, Cellule E. coli per trasformazione, Cellule competenti per clonaggio

Q: What is the transformation efficiency of CVX5α cells?
A: ≥1 × 10⁷ CFU/µg DNA with pUC18 plasmid.

Q: Are they suitable for blue/white screening?
A: Yes, compatible with X-gal or Bluo-gal selection.

Q: What storage is required?
A: Store at –80 ºC, avoid freeze-thaw cycles.