pSpark® TA Cloning Kit – High-efficiency TA vector system for A-tailed PCR products

pSpark® TA Done

Name: pSpark® TA Done
SKU: C0021
Price: 184.10 EUR
Availability: InStock

High-efficiency TA cloning kit for direct ligation of A-tailed PCR products generated by Taq polymerase.

pSpark® TA-Done Cloning Kit provides a fast, simple, and high-efficiency system for direct cloning of PCR products generated by Taq DNA polymerase. Its optimized TA vector features 3′-T overhangs that enable quick ligation of A-tailed fragments without additional enzymatic modification.

The kit ensures maximum transformation efficiency, minimal background, and stable insert maintenance, making it ideal for routine cloning, sequencing, and construct verification. Its straightforward workflow allows researchers to move seamlessly from PCR to plasmid in a single step.

Have questions? Contact us

20 rxn

Price: 184.10 €

SKU: C0021 Categories: DNA Cloning, TA Cloning Kits

Detailed information:

Advantages & Features

High cloning efficiency: Optimized for A-tailed PCR products from Taq polymerase.
Direct ligation workflow: No need for polishing or additional modification steps.
Low background: Proprietary vector design minimizes self-ligation events.
Fast and simple: PCR-to-clone in less than 30 minutes.
Stable inserts: Ensures integrity and high propagation efficiency.
Broad compatibility: Works with standard E. coli cloning strains.

Specifications

Includes

– 20 rxn pSpark® TA Done (20ng/µL)
– 20 µL T4 DNA Ligase (5 Weiss U/µL)
– 100 µL T4 DNA Ligase Buffer (10x)
– 5 µL Insert Control 600bp control insert
– 150 µL PEG 6000 solution (10x)

Download documentation

Datasheet

MSDS

Application Note

Selection Guide

Applications

TA cloning of PCR products.
DNA fragment subcloning.
Sequencing and mutagenesis workflows.
Construct generation and plasmid design.
Routine molecular cloning applications.

Tables & Figures

Quality Control

Each batch is validated for ligation efficiency, transformation rate, and insert stability, ensuring consistent and reproducible cloning results.

Advice

Use PCR products generated with non-proofreading Taq DNA polymerases.
Keep ligation reagents cold during reaction setup.
Always include a control ligation to evaluate vector background.

Storage, Shipping & Guarantee

Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).

Citations

Alvarez, J. B., Castellano, L., & Guzmán, C. (2025). Molecular characterization of the variability of the GLU-Hch1 gene in Hordeum chilense. Applied Food Research, 100784.
Sanchez-Briñas, A., Duran-Ruiz, C., Astola, A., Arroyo, M. M., Raposo, F. G., Valle, A., & Bolivar, J. (2023). ZNF330/NOA36 interacts with HSPA1 and HSPA8 and modulates cell cycle and proliferation in response to heat shock in HEK293 cells. Biology Direct, 18(1), 1-14.
Cabrera, A., Castellano, L., Recio, R., & Alvarez, J. B. (2019). Chromosomal location and molecular characterization of three grain hardness genes in Agropyron cristatum. Euphytica, 215(10), 165.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

FAQs

Q1: What type of PCR products are compatible with pSpark® TA-Done?
A-tailed fragments generated by non-proofreading Taq DNA polymerases.

Q2: How does it differ from pSpark® III or IV?
pSpark® TA-Done is specifically designed for A-overhang PCR fragments, while pSpark® III and IV are for blunt-ended products.

Q3: What transformation efficiency can I expect?
Typically higher than 10⁶ cfu/µg DNA using standard E. coli strains.