pSpark® TA

– 20 rxn pSpark® TA (50 ng/µL)
– 20 µL T4 DNA Ligase (5 Weiss U/µL)
– 100 µL T4 DNA Ligase Buffer (10x)
– 5 µL Insert Control 600bp control insert

• Direct TA cloning of PCR products.

• DNA fragment subcloning.

• Sequencing and site-directed mutagenesis.

• Plasmid construction and vector design.

• Routine molecular cloning workflows.

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• Each batch is tested for ligation efficiency, transformation rate, and insert stability to guarantee reproducible cloning performance.

• Use PCR products obtained with Taq or other non-proofreading polymerases.

• Keep ligation reagents on ice during preparation.

• Include negative controls to assess background colony formation.

• Shipped in: Gel Pack.
• Storage: -20 ºC (NON Frost-Free Freezer).

• Sanchez-Briñas, A., Duran-Ruiz, C., Astola, A., Arroyo, M. M., Raposo, F. G., Valle, A., & Bolivar, J. (2023). ZNF330/NOA36 interacts with HSPA1 and HSPA8 and modulates cell cycle and proliferation in response to heat shock in HEK293 cells. Biology Direct, 18(1), 1-14.
• Huertas-García, A. B., Guzmán, C., Tabbita, F., & Alvarez, J. B. (2022). Allelic Variation of Puroindolines Genes in Iranian Common Wheat Landraces. Agriculture, 12(8), 1196.
• Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
• Morales de los Ríos Martín, L. (2022). Influencia del transportador de nitrato NRT1. 4/NPF6. 2 en la nutrición de potasio en Arabidopsis thaliana.
• Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
• Álvarez Escribano, I. (2019). Caracterización de los sRNAs reguladores NsrR1 y NsiR3 de la cianobacteria Nostoc sp. PCC 7120.
• Moreno Cabezuelo, J. Á. (2019). Glucose uptake in marine cyanobacteria: regulation, expression of the transporter and effects on the proteome and metabolome.
• Alvarez, J. B., Castellano, L., Recio, R., & Cabrera, A. (2019). Wx Gene in Hordeum chilense: Chromosomal Location and Characterisation of the Allelic Variation in the Two Main Ecotypes of the Species. Agronomy, 9(5), 261.
• Lindo, L., McCormick, S. P., Cardoza, R. E., Kim, H. S., Brown, D. W., Alexander, N. J., … & Gutiérrez, S. (2018). Role of Trichoderma arundinaceum tri10 in regulation of terpene biosynthetic genes and in control of metabolic flux. Fungal Genetics and Biology.
• Huyghe, J. (2017). Selection of reference genes for gene expression studies in Lactic acid bacteria.
• Rivas, A. J., von Hoven, G., Neukirch, C., Meyenburg, M., Qin, Q., Füser, S., … & Husmann, M. (2015). Phobalysin, a Small β-Pore-Forming Toxin of Photobacterium damselae subsp. damselae. Infection and immunity, 83(11), 4335-4348.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

TA cloning vector, PCR cloning vector, T vector plasmid, DNA cloning plasmid, TA cloning plasmid

Also known as:

• Spanish: Vector de clonación TA, Vector T para clonación, Plásmido de clonación
• French: Vecteur de clonage TA, Vecteur T pour clonage, Plasmide de clonage
• German: TA-Klonierungsvektor, T-Vektor für Klonierung, Klonierungsplasmid
• Italian: Vettore di clonaggio TA, Vettore T per clonaggio, Plasmide di clonaggio

Q1: What PCR products are compatible with this kit?
PCR fragments generated by non-proofreading Taq polymerases with A-overhangs.

Q2: How is pSpark® TA different from pSpark® TA-Done?
pSpark® TA is the standard version, while pSpark® TA-Done includes further optimized reagents for higher efficiency and lower background.

Q3: Can it be used for high-throughput cloning?
Yes. Its high efficiency and simplicity make it suitable for automated workflows.