pSpark® TA

Name: pSpark® TA
SKU: C0020
Price: 162.60 EUR
Availability: InStock

Fast and reliable TA cloning kit for direct ligation of A-tailed PCR fragments.

pSpark® TA Cloning Kit offers a fast, easy, and efficient system for cloning PCR products generated by Taq DNA polymerase. The vector includes 3′-T overhangs that enable direct ligation of A-tailed fragments without the need for restriction enzymes or polishing steps, saving valuable time in routine molecular biology workflows.

Its optimized design ensures high transformation efficiency and low background, providing consistent and reproducible results. This kit is ideal for routine cloning, sequencing, and mutagenesis, allowing researchers to move directly from PCR to plasmid in less than one hour.

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20 rxn

Price: 162.60 €

SKU: C0020 Categories: DNA Cloning, TA Cloning Kits

Detailed information:

Advantages & Features

High cloning efficiency: Optimized for A-tailed PCR products from Taq polymerase.
Direct ligation workflow: No post-PCR modification or enzymatic polishing needed.
Low background: Proprietary vector design reduces self-ligation.
Fast and simple: PCR-to-vector workflow in under 60 minutes.
Stable insert maintenance: Ensures plasmid integrity after transformation.
Broad host compatibility: Works with common E. coli cloning strains.

Specifications

Includes

– 20 rxn pSpark® TA (50 ng/µL)
– 20 µL T4 DNA Ligase (5 Weiss U/µL)
– 100 µL T4 DNA Ligase Buffer (10x)
– 5 µL Insert Control 600bp control insert

Download documentation

Datasheet

MSDS

Application Note

Selection Guide

Applications

Direct TA cloning of PCR products.
DNA fragment subcloning.
Sequencing and site-directed mutagenesis.
Plasmid construction and vector design.
Routine molecular cloning workflows.

Tables & Figures

Quality Control

Each batch is tested for ligation efficiency, transformation rate, and insert stability to guarantee reproducible cloning performance.

Advice

Use PCR products obtained with Taq or other non-proofreading polymerases.
Keep ligation reagents on ice during preparation.
Include negative controls to assess background colony formation.

Storage, Shipping & Guarantee

Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).

Citations

Sanchez-Briñas, A., Duran-Ruiz, C., Astola, A., Arroyo, M. M., Raposo, F. G., Valle, A., & Bolivar, J. (2023). ZNF330/NOA36 interacts with HSPA1 and HSPA8 and modulates cell cycle and proliferation in response to heat shock in HEK293 cells. Biology Direct, 18(1), 1-14.
Huertas-García, A. B., Guzmán, C., Tabbita, F., & Alvarez, J. B. (2022). Allelic Variation of Puroindolines Genes in Iranian Common Wheat Landraces. Agriculture, 12(8), 1196.
Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
Morales de los Ríos Martín, L. (2022). Influencia del transportador de nitrato NRT1. 4/NPF6. 2 en la nutrición de potasio en Arabidopsis thaliana.
Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
Álvarez Escribano, I. (2019). Caracterización de los sRNAs reguladores NsrR1 y NsiR3 de la cianobacteria Nostoc sp. PCC 7120.
Moreno Cabezuelo, J. Á. (2019). Glucose uptake in marine cyanobacteria: regulation, expression of the transporter and effects on the proteome and metabolome.
Alvarez, J. B., Castellano, L., Recio, R., & Cabrera, A. (2019). Wx Gene in Hordeum chilense: Chromosomal Location and Characterisation of the Allelic Variation in the Two Main Ecotypes of the Species. Agronomy, 9(5), 261.
Lindo, L., McCormick, S. P., Cardoza, R. E., Kim, H. S., Brown, D. W., Alexander, N. J., … & Gutiérrez, S. (2018). Role of Trichoderma arundinaceum tri10 in regulation of terpene biosynthetic genes and in control of metabolic flux. Fungal Genetics and Biology.
Huyghe, J. (2017). Selection of reference genes for gene expression studies in Lactic acid bacteria.
Rivas, A. J., von Hoven, G., Neukirch, C., Meyenburg, M., Qin, Q., Füser, S., … & Husmann, M. (2015). Phobalysin, a Small β-Pore-Forming Toxin of Photobacterium damselae subsp. damselae. Infection and immunity, 83(11), 4335-4348.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

FAQs

Q1: What PCR products are compatible with this kit?
PCR fragments generated by non-proofreading Taq polymerases with A-overhangs.

Q2: How is pSpark® TA different from pSpark® TA-Done?
pSpark® TA is the standard version, while pSpark® TA-Done includes further optimized reagents for higher efficiency and lower background.

Q3: Can it be used for high-throughput cloning?
Yes. Its high efficiency and simplicity make it suitable for automated workflows.