HigherPurity™ Saliva DNA Extraction Mini Spin Kit

Fast, simple, and reliable method for isolating high-quality genomic DNA from human or animal saliva using silica mini spin columns.

HigherPurity™ Saliva DNA Extraction Mini Spin Kit offers an easy, rapid, and highly reproducible system for purifying genomic DNA from saliva samples.

The extraction principle relies on the ability of DNA to bind to silica membranes under chaotropic conditions, ensuring efficient removal of proteins, inhibitors, and impurities.
The protocol eliminates the need for hazardous organic solvents such as phenol or chloroform, delivering pure and intact genomic DNA suitable for all molecular biology applications.

This kit provides a reliable solution for routine DNA isolation from both human and animal saliva, ideal for PCR-based genotyping, sequencing, or molecular diagnostics research.

The kit is compatible with both fresh and preserved saliva samples, including those stabilized using the Saliva DNA Sample Collection & Stabilization Kit.

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Price: 138.40 544.40 

SKU: AN0098 Categories: ,

Detailed information:

Advantages & Features

  • Easy and fast protocol: complete extraction in just a few minutes.
  • High-quality genomic DNA: pure and ready-to-use in all molecular biology applications.
  • Reproducible performance: consistent recovery of high-molecular-weight DNA.
  • Safe and convenient: avoids phenol/chloroform extraction.
  • Efficient yields: typically 3–12 µg depending on saliva sample type and volume.
  • Mini spin format: user-friendly columns suitable for manual or small-scale workflows.

Specifications

Includes

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 50 Collection tubes (2 mL)
– 100 mL PBS (1x)
– 20 mL BLY Buffer
– 15 mg Proteinase K
– 30 mL WB1 Buffer
– 6 mL WB2 Buffer
– 10 mL EB Buffer

Download documentation

Datasheet
MSDS

Applications

gDNA Purified DNA suitable for all common Molecular Biology applications, such as:

  • PCR and qPCR
  • Cloning.
  • DNA sequencing.
  • Southern blot analysis.
  • Genotyping and molecular marker studies (RUO).

Tables & Figures

Quality Control

– Tested on a lot-to-lot basis by isolating total DNA from Human Sample Saliva.
– DNA purified is analysed by:

• Spectrophotometer: Ratio 260/ 280 (1.6-1.8)
• Agarose Gel Electrophoresis.

Advice

  • Recommendations: If any kit reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves, and allow to cool to room temperature before use.

Storage, Shipping & Guarantee

  • Shipped at: Ambient Temperature.
  • Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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