Clean-Easy™ Agarose Purification Kit

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 50 Collection tubes (2 mL)
– 60 mL QG Buffer
– 11.25 mL PE Buffer
– 10 mL EB Buffer

• Recovery of DNA fragments from agarose gels after PCR or restriction digestion.

• Preparation of DNA for PCR, sequencing, cloning, and Southern blotting.

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• Tested with 0.5 kb DNA fragment excised from 2% agarose.

• Purity confirmed (A260/A280 ratio: 1.8–2.0).

• Integrity checked by agarose gel electrophoresis.

• Excise gel slices with minimal UV exposure to avoid DNA damage.

• Ensure proper melting of gel slice in QG Buffer for maximum yield.

• Shipped in: Ambient Temperature.
• Storage: All components can be stored at Room Temperature (15–25 °C).

• Stefanova, P., Georgieva, A., Brazkova, M., Baldzhieva, R., Goranov, B., Blazheva, D., … & Angelova, G. (2025). Molecular Identification, Mycelial Growth Kinetics, and Antimicrobial Potential of Newly Isolated Medicinal Mushroom Fomitopsis pinicola from Bulgaria. Journal of Fungi, 11(10), 727.
• D’Anza, E., Albarella, S., Cascone, I., Pugliano, M., Ciotola, F., & Peretti, V. (2025). A fast molecular tool for sexing Gibber Italicus, song thrush, and redwing birds. Journal of Veterinary Diagnostic Investigation, 10406387251323555.
• OSTUNI, Angela, et al. Circulation of small ruminant lentivirus in endangered goat and sheep breeds of Southern Italy. Heliyon, 2024.
• Angelova, G., Stefanova, P., Brazkova, M., & Krastanov, A. (2023). Molecular and morphological characterization of Xylaria karsticola (Ascomycota) isolated from the fruiting body of Macrolepiota procera (Basidiomycota) from Bulgaria. Plos one, 18(6), e0287679.
• Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
• Petkova, Mariana, et al. “Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs.” Microorganisms 9.7 (2021): 1346.
• Nistal-García, A., García-García, P., García-Girón, J., Borrego-Ramos, M., Blanco, S., & Bécares, E. (2020). DNA metabarcoding and morphological methods show complementary patterns in the metacommunity organization of lentic epiphytic diatoms. Authorea Preprints.
• Romero, I., Casillas-Gonzalez, A. C., Carrazana-Villalba, S. J., Escribano, M. I., Merodio, C., & Sanchez-Ballesta, M. T. (2018). Impact of high CO2 levels on heat shock proteins during postharvest storage of table grapes at low temperature. Functional in vitro characterization of VVIHSP18. 1. Postharvest Biology and Technology, 145, 108-116.
• Moreno, B., Vivas, A., Nogales, R., Macci, C., Masciandaro, G., & Benitez, E. (2009). Restoring biochemical activity and bacterial diversity in a trichloroethylene-contaminated soil: the reclamation effect of vermicomposted olive wastes. Environmental Science and Pollution Research, 16(3), 253-264.
• Vivas, A., Moreno, B., Garcia-Rodriguez, S., & Benítez, E. (2009). Assessing the impact of composting and vermicomposting on bacterial community size and structure, and microbial functional diversity of an olive-mill waste. Bioresource Technology, 100(3), 1319-1326.
• Moreno, B., Nogales, R., Macci, C., Masciandaro, G., & Benitez, E. (2011). Microbial eco-physiological profiles to estimate the biological restoration of a trichloroethylene-contaminated soil. Ecological Indicators, 11(6), 1563-1571.
• Moreno, B., Cañizares, R., Nuñez, R., & Benitez, E. (2013). Genetic diversity of bacterial β-glucosidase-encoding genes as a function of soil management. Biology and fertility of soils, 49(6), 735-745.
• Rubio, L. A., Peinado, M. J., Echávarri, A., Ruiz, R., Suárez-Pereira, E., Mellet, C. O., & Fernández, J. M. G. (2014). A Di-D-Fructose Dianhydride-Enriched Caramel Modulates Pig Fecal Microbiota Composition. Advances in Microbiology, 2014.
• Cañizares, R., Moreno, B., & Benitez, E. (2012). Biochemical characterization with detection and expression of bacterial β-glucosidase encoding genes of a Mediterranean soil under different long-term management practices. Biology and Fertility of Soils, 48(6), 651-663.
• Cañizares, R., Benitez, E., & Ogunseitan, O. A. (2011). Molecular analyses of β-glucosidase diversity and function in soil. European Journal of Soil Biology, 47(1), 1-8.
• Vivas, A., Moreno, B., Garcia-Rodriguez, S., & Benítez, E. (2009). Assessing the impact of composting and vermicomposting on bacterial community size and structure, and microbial functional diversity of an olive-mill waste. Bioresource Technology, 100(3), 1319-1326.
• Sánchez-Castro, I., Amador-García, A., Moreno-Romero, C., López-Fernández, M., Phrommavanh, V., Nos, J., … & Merroun, M. L. (2017). Screening of bacterial strains isolated from uranium mill tailings porewaters for bioremediation purposes. Journal of environmental radioactivity, 166, 130-141.
• Peinado, M. J., Ruiz, R., Echávarri, A., & Rubio, L. A. (2012). Garlic derivative propyl propane thiosulfonate is effective against broiler enteropathogens in vivo. Poultry science, 91(9), 2148-2157.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q: What is the minimum fragment size recoverable?
A: ≥100 bp.

Q: What is the typical recovery yield?
A: >80% from 0.7–1% agarose gels.

Q: How long does the procedure take?
A: Approximately 5 minutes.