HigherPurity™ Bacterial DNA Isolation Kit

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 50 Collection tubes
– 15 mL BR-1 Buffer
– 20 mL BLU Buffer
– 30 mL WB1 Buffer
– 30 mL WB2 Buffer
– 15 mL EB Buffer
– 30 mg Proteinase K
– 25 mg Lysozyme

Purified DNA suitable for all common Molecular Biology applications, such as:

• Genomic DNA isolation from Gram-negative & Gram-positive bacteria
• PCR & qPCR
• Sequencing
• Southern blotting
• Hybridization assays
• Genotyping & epidemiological studies (RUO)

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• Tested on a lot-to-lot basis by isolating total DNA from E. coli.
• Tested for the isolation of any Plasmid DNA from transformed E.coli.
• The Quality of Purified DNA is analysed by:
  • • Ratio 260/ 280 (1.8-2).
    • Agarose Gel Electrophoresis.
    • Digestion with Restriction Endonucleases.

• If any kit reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves, and allow to cool to room temperature before use.
• Avoid overloading columns to maintain DNA quality
• Ensure complete resuspension of pellets before lysis.

• Shipped at: Ambient Temperature.
• Storage: All components can be stored at Room Temperature, except Proteinase K and Lysozyme (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

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• Shahayeva, M. (2025). Influència de la infecció per Helicobacter pylori en la microbiota gàstrica.
• Biada, I., Tiezzi, F., Ibáñez-Escriche, N., Luz García, M., Argente, M. J., & Santacreu, M. A. (2025). Differential intestinal microbiome response to heat stress in two rabbit maternal lines: A comparative analysis using Random Forest, BayesC, and PLS-DA. Journal of Animal Science, skaf206.
• Díaz-Rullo Edreira, S., Vasiliadou, I. A., Prado, A., Espada, J. J., Wattiez, R., Leroy, B., … & Puyol, D. (2024). Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis. Communications Biology, 7(1), 1526.
• Petkova, M., & Dimova, M. (2024). Biological Control of Lettuce Drop (Sclerotinia minor Jagger) Using Antagonistic Bacillus Species. Applied Microbiology, 4(3), 1283-1293.
• Iungin, O.; Prekrasna-Kviatkovska, Y.; Kalinichenko, O.; Moshynets, O.; Potters, G.; Sidorenko, M.; Savchuk, Y.; Mickevičius, S. Endophytic Bacterial Biofilm-Formers Associated with Antarctic Vascular Plants. Microorganisms 2024, 12, 1938
• Aiddi, A., Zerdani, I., Khazaz, A., Benzaarate, I., Mguild, H., Bourjilat, F., & Nayme, K. (2024). First report of blaOXA-48 producing Klebsiella pneumoniae isolates from wastewater in Morocco. Journal of Public Health in Africa, 15(1), 3.
• Taylor, A.; Griffin, C.; Arrington, K.; Barragan, J.; Cervantes, J. (2024) IL-26 Increases Sensing of Borrelia burgdorferi DNA by Human Toll-like Receptor 9. Microbiol. Res. 15, 1319-1325.
• Edreira, S. D. R., Vasiliadou, I., Prado, A., Espada, J., Wattiez, R., Leroy, B., … & Puyol, D. (2023). Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis.
• Ruiz, M. J., Salatti-Dorado, J. A., Cardador, M. J., Frizzo, L., Jordano, R., Arce, L., & Medina, L. M. (2023). Relationship between Volatile Organic Compounds and Microorganisms Isolated from Raw Sheep Milk Cheeses Determined by Sanger Sequencing and GC–IMS. Foods, 12(2), 372.
• Chong, E. T. J., Png, K. K., Abidueva, E., Zaitseva, S., Sun, C., & Lee, P. C. (2023). Dataset of the complete genome of Streptomyces cavourensis strain 2BA6PGT isolated from sediment from the bottom of the salt lake Verkhnee Beloe (Buryatia, Russia). Data in Brief, 108877.
• San Martín, J., Puyol, D., Segura, Y., Melero, J. A., & Martínez, F. (2023). A novel photoanaerobic process as a feasible alternative to the traditional aerobic treatment of refinery wastewater. Journal of Water Process Engineering, 51, 103352.
• Kajsikova, M., Kajsik, M., Drahovska, H., & Bukovska, G. (2022). Complete genome sequence of the industrial l-lysine production strain [Brevibacterium] flavum CCM 251. Biologia, 1-6.
• Gonzalez-Pimentel, J. L., Domínguez-Moñino, I., Jurado, V., Caldeira, A. T., & Saiz-Jimenez, C. (2021). Aerobiology from an Inactive Pyrite Mine: the Genome Sequence of the Airborne Pseudomonas sp. Strain L5B5. Microbiology Resource Announcements, 10(48), e01029-21.
• Petkova, Mariana, et al. “Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs.” Microorganisms 9.7 (2021): 1346.
• Ferreira, M. J., Cunha, A., Figueiredo, S., Faustino, P., Patinha, C., Silva, H., & Sierra-Garcia, I. N. (2021). The Root Microbiome of Salicornia ramosissima as a Seedbank for Plant-Growth Promoting Halotolerant Bacteria. Applied Sciences, 11(5), 2233.
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• Tkacova, A., Orieskova, M., Halgasova, N., Bocanova, L., & Bukovska, G. (2019). Identification of Brevibacterium flavum genes related to receptors involved in bacteriophage BFK20 adsorption. Virus Research, 197775.
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This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.