HigherPurity™ Bacterial DNA Isolation Kit

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 50 Collection tubes (2 mL)
– 15 mL BR-1 Buffer
– 20 mL BLU Buffer
– 30 mL WB1 Buffer
– 6 mL WB2 Buffer
– 10 mL EB Buffer
– 30 mg Proteinase K
– 25 mg Lysozyme
– 1 mL RNase A Solution (10 mg/ml)

Purified DNA suitable for all common Molecular Biology applications, such as:

• Genomic DNA isolation from Gram-negative & Gram-positive bacteria
• PCR & qPCR
• Sequencing
• Southern blotting
• Hybridization assays
• Genotyping & epidemiological studies (RUO)

t

• Tested on a lot-to-lot basis by isolating total DNA from E. coli.
• Tested for the isolation of any bacterial DNA from transformed E.coli.
• The Quality of Purified DNA is analysed by:
  • • Ratio 260/ 280 (1.8-2).
    • Agarose Gel Electrophoresis.
    • Digestion with Restriction Endonucleases.

• If any kit reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves, and allow to cool to room temperature before use.
• Avoid overloading columns to maintain DNA quality
• Ensure complete resuspension of pellets before lysis.

• Shipped at: Ambient Temperature.
• Storage: All components can be stored at Room Temperature, except Proteinase K and Lysozyme (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

• Shaji, A., Ramachandran, A. K., Chandrasekaran, N., Savarimalai, K. C., & Adhira, R. (2026). A cross-sectional metagenomic analysis of the microbial ecology in symptomatic apical periodontitis–An in vivo study. Journal of Conservative Dentistry and Endodontics, 29(1), 60-64.
• Mërtiri, I., Mihalcea, L., Aprodu, I., Cioromila, A. C., Turturică, M., Râpeanu, G., … & Grigore-Gurgu, L. (2025). Phytochemical profile and antilisterial activity of Salvia officinalis Supercritical Fluid Extract. Applied Food Research, 101379.
• Shahayeva, M. (2025). Influència de la infecció per Helicobacter pylori en la microbiota gàstrica.
• Biada, I., Tiezzi, F., Ibáñez-Escriche, N., Luz García, M., Argente, M. J., & Santacreu, M. A. (2025). Differential intestinal microbiome response to heat stress in two rabbit maternal lines: A comparative analysis using Random Forest, BayesC, and PLS-DA. Journal of Animal Science, skaf206.
• Díaz-Rullo Edreira, S., Vasiliadou, I. A., Prado, A., Espada, J. J., Wattiez, R., Leroy, B., … & Puyol, D. (2024). Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis. Communications Biology, 7(1), 1526.
• Petkova, M., & Dimova, M. (2024). Biological Control of Lettuce Drop (Sclerotinia minor Jagger) Using Antagonistic Bacillus Species. Applied Microbiology, 4(3), 1283-1293.
• Iungin, O.; Prekrasna-Kviatkovska, Y.; Kalinichenko, O.; Moshynets, O.; Potters, G.; Sidorenko, M.; Savchuk, Y.; Mickevičius, S. Endophytic Bacterial Biofilm-Formers Associated with Antarctic Vascular Plants. Microorganisms 2024, 12, 1938
• Aiddi, A., Zerdani, I., Khazaz, A., Benzaarate, I., Mguild, H., Bourjilat, F., & Nayme, K. (2024). First report of blaOXA-48 producing Klebsiella pneumoniae isolates from wastewater in Morocco. Journal of Public Health in Africa, 15(1), 3.
• Taylor, A.; Griffin, C.; Arrington, K.; Barragan, J.; Cervantes, J. (2024) IL-26 Increases Sensing of Borrelia burgdorferi DNA by Human Toll-like Receptor 9. Microbiol. Res. 15, 1319-1325.
• Edreira, S. D. R., Vasiliadou, I., Prado, A., Espada, J., Wattiez, R., Leroy, B., … & Puyol, D. (2023). Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis.
• Ruiz, M. J., Salatti-Dorado, J. A., Cardador, M. J., Frizzo, L., Jordano, R., Arce, L., & Medina, L. M. (2023). Relationship between Volatile Organic Compounds and Microorganisms Isolated from Raw Sheep Milk Cheeses Determined by Sanger Sequencing and GC–IMS. Foods, 12(2), 372.
• Chong, E. T. J., Png, K. K., Abidueva, E., Zaitseva, S., Sun, C., & Lee, P. C. (2023). Dataset of the complete genome of Streptomyces cavourensis strain 2BA6PGT isolated from sediment from the bottom of the salt lake Verkhnee Beloe (Buryatia, Russia). Data in Brief, 108877.
• San Martín, J., Puyol, D., Segura, Y., Melero, J. A., & Martínez, F. (2023). A novel photoanaerobic process as a feasible alternative to the traditional aerobic treatment of refinery wastewater. Journal of Water Process Engineering, 51, 103352.
• Kajsikova, M., Kajsik, M., Drahovska, H., & Bukovska, G. (2022). Complete genome sequence of the industrial l-lysine production strain [Brevibacterium] flavum CCM 251. Biologia, 1-6.
• Gonzalez-Pimentel, J. L., Domínguez-Moñino, I., Jurado, V., Caldeira, A. T., & Saiz-Jimenez, C. (2021). Aerobiology from an Inactive Pyrite Mine: the Genome Sequence of the Airborne Pseudomonas sp. Strain L5B5. Microbiology Resource Announcements, 10(48), e01029-21.
• Petkova, Mariana, et al. “Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs.” Microorganisms 9.7 (2021): 1346.
• Ferreira, M. J., Cunha, A., Figueiredo, S., Faustino, P., Patinha, C., Silva, H., & Sierra-Garcia, I. N. (2021). The Root Microbiome of Salicornia ramosissima as a Seedbank for Plant-Growth Promoting Halotolerant Bacteria. Applied Sciences, 11(5), 2233.
• Ruiz, M. J., Colello, R., Etcheverría, A. I., & Medina, L. M. (2019). Secuenciación y evaluación del efecto antimicrobiano de bacterias acidolácticas frente a cepas de Salmonella Tiphymurium. Archivos de Zootecnia, 68(264), 534-538.
• Tkacova, A., Orieskova, M., Halgasova, N., Bocanova, L., & Bukovska, G. (2019). Identification of Brevibacterium flavum genes related to receptors involved in bacteriophage BFK20 adsorption. Virus Research, 197775.
• Valiente, N., Carrey, R., Otero, N., Gutiérrez-Villanueva, M. A., Soler, A., Sanz, D., … & Gómez-Alday, J. J. (2017). Tracing sulfate recycling in the hypersaline Pétrola Lake (SE Spain): A combined isotopic and microbiological approach. Chemical Geology, 473, 74-89.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Bacterial DNA extraction kit, Genomic DNA isolation bacteria, DNA purification bacterial cells, Bacterial DNA isolation kit, DNA extraction bacteria samples

Also known as:

• Spanish: Kit de extracción de ADN bacteriano, Aislamiento de ADN genómico bacteriano, Purificación de ADN bacteriano
• French: Kit d’extraction ADN bactérien, Isolation ADN génomique bactéries, Purification ADN bactérien
• German: DNA-Extraktionskit für Bakterien, Genomische DNA-Isolation Bakterien, DNA-Reinigung Bakterien
• Italian: Kit estrazione DNA batterico, Isolamento DNA genomico batteri, Purificazione DNA batterico