HigherPurity™ FFPE DNA Isolation Kit

Name: HigherPurity™ FFPE DNA Isolation Kit
SKU: AN0160
Availability: InStock

HigherPurity™ FFPE DNA Isolation Kit provides a fast, safe, and xylene-free method for extracting high-quality DNA from formalin-fixed, paraffin-embedded (FFPE) tissues.
Its optimized two-phase protocol (Deparaffinization + DNA Extraction) ensures pure, inhibitor-free DNA, ideal for PCR, qPCR, NGS, and STR analysis, even from long-archived samples.

HigherPurity™ FFPE DNA Isolation Kit provides an easy, rapid, and highly efficient method for extracting and purifying DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples, the most common form of long-term preservation for archived pathological specimens.

The extraction process consists of two optimized phases:
1) Deparaffinization, using a proprietary non-toxic buffer that completely dissolves paraffin without the use of flammable or malodorous solvents such as xylene or d-limonene.
2) DNA Isolation, delivering high-quality, inhibitor-free DNA ideal for sensitive downstream molecular applications.

The result is high-purity DNA suitable for reliable PCR, qPCR, NGS, or STR analysis, even from decades-old FFPE samples.

Have questions? Contact us

Price: 210.48 € – 823.25 €

size

50 rxn

250 rxn

SKU: AN0160 Categories: DNA Extraction Kits, DNA & RNA Extraction

Detailed information:

Advantages & Features

High-quality DNA: suitable for all molecular biology downstream applications.
Fast and simple: total extraction process completed in less than 2 hours 45 min with minimal hands-on time.
Safe and solvent-free: eliminates the need for xylene or other toxic organic solvents.
High purity and yield: complete removal of contaminants and PCR inhibitors.
Low elution volume: concentrated DNA recovery between 15–30 µL.
Long-term preservation: optimized for stable extraction from archived FFPE samples.
Comprehensive kit: includes all reagents for both Deparaffinization and DNA Extraction phases.

Specifications

Includes

Includes for 50 rxn:
– 30 mL Deparaffinization solution
– 10 mL Tissue Lysis Buffer
– 15 mL Lysis/ Binding Buffer
– 2 x 30 mg Proteinase K
– 18 mL Desinhibition Buffer
– 10 mL Wash Buffer
– 10 mL Elution Buffer
– 50 units MicroSpin Columns
– 100 units Collection tubes

Download documentation

Datasheet

MSDS

Applications

Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples.
Isolation of DNA from fresh and archived FFPE samples.
Isolation of DNA from specimen of object slides.
Typical downstream applications such as:
- PCR & qPCR
- Next-Generation Sequencing
- Short Tandem Repeat (STR) analysis (RUO)
- Mutation screening and genotyping (RUO

Tables & Figures

Quality Control

DNA isolated from tissue slice Paraffin-embedded (10 µm) quantified by Spectrophotometer and analysed by PCR.

Advice

Storage, Shipping & Guarantee

Shipped in: Ambient Temperature.
Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

Citations

Sevilla, A., Morales, M. C., Ezkurra, P. A., Rasero, J., Velasco, V., Cancho-Galan, G., … & Asumendi, A. (2020). BRAF V600E mutational load as a prognosis biomarker in malignant melanoma. PLOS ONE, 15(3), e0230136.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.