HigherPurity™ Soil DNA Isolation Kit

Name: HigherPurity™ Soil DNA Isolation Kit
SKU: AN0140
Availability: InStock

For Efficient, Easy & High-Purity DNA Isolation from Diverse Soil Types

HigherPurity™ Soil DNA Isolation Kit enables rapid and efficient extraction of high-quality DNA from a wide range of soil samples using bead-beating and silica membrane purification.
The optimized buffer system efficiently removes humic acids, polysaccharides, and PCR inhibitors, providing ready-to-use DNA in under 1 hour, ideal for PCR, restriction analysis, and sequencing.

It removes inhibitors such as humic acids, producing high-purity DNA.

The extracted DNA is ready for PCR and other molecular analyses, ensuring reliable performance across diverse soil types.

Have questions? Contact us

Price: 139.22 € – 495.49 €

size

50 rxn

100 rxn

250 rxn

SKU: AN0140 Categories: DNA Extraction Kits, DNA & RNA Extraction

Detailed information:

Advantages & Features

High-quality DNA: suitable for all molecular biology downstream applications.
Rapid protocol: obtain ready-to-use DNA in approximately 58 minutes.
Safe and convenient: eliminates toxic phenol/chloroform extractions.
High purity: efficiently removes humic acids, polysaccharides, phenolic compounds, and other PCR inhibitors.
Versatile sample size: processes 0.2–1 g of soil per preparation.
Efficient lysis: bead-beating ensures complete disruption of microbial cells.
Reliable performance: optimized buffers maintain reproducibility across diverse soil types.

Specifications

Includes

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 100 Collection tubes (2 mL)
– 50 Dry bead tube
– 12 g Glass Bead
– 40 mL Lysis Solution 1 (LS1)
– 20 mL WB1 Buffer
– 35 mL EB Buffer
– 15 mL Inhibitor Removal Buffer (IR-Buffer)
– 50 microcentrifuge tube (1.5 mL)
– 15 mL Buffer A
– 25 mL Buffer

Download documentation

Datasheet

MSDS

Applications

Purified DNA suitable for all common Molecular Biology applications, such as:

PCR and qPCR amplification.
Restriction enzyme digestion.
Automated sequencing.
Metagenomic and microbial diversity analysis.
Southern Blots.

Tables & Figures

Quality Control

Tested for isolation of DNA from soil sample.
The quantity and quality of purified DNA attend to:
- Ratio 260/ 280 (1.8-2).
- Agarose gel electrophoresis.

Advice

Precautions: Please wear gloves when using this product. Avoid all skin contact with kit reagents. In case of contact, wash thoroughly with water.

Storage, Shipping & Guarantee

Shipped in: Ambient Temperature.
Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

Citations

Puyol, D., Allegue, L., Ventura, M., Vlaeminck, S., Melero, J., & Leroy, B. (2025). Purple phototrophic bacteria release crotonate as metabolic overflow pathway to complement other redox-balancing routes.
Sáez‐Sandino, T., Reich, P. B., Maestre, F. T., Cano‐Díaz, C., Stefanski, A., Bermudez, R., … & Trivedi, P. (2025). A Large Fraction of Soil Microbial Taxa Is Sensitive to Experimental Warming. Global Change Biology, 31(5), e70231.
Cortijo-Alfonso, M. E., Laghouaouta, H., Pena, R. N., Martínez-Subirà, M., Yuste, S., Rubió-Piqué, L., & Piñol, C. (2025). Gut Microbiota Modulation And Inflammation Mitigation In a Murine Model Through A Hull-Less And Purple Grain Barley Genotype. Food & Function.
Azouggagh, L., Ibáñez-Escriche, N., Martínez-Álvaro, M., Varona, L., Casellas, J., Negro, S., & Casto-Rebollo, C. (2025). Characterization of microbiota signatures in Iberian pig strains using machine learning algorithms. Animal Microbiome, 7(1), 13.
Withey, Z., & Gweon, H. S. (2024). Longitudinal bacterial community dynamics and sodium hypochlorite intervention in a newly built university building. Science of The Total Environment, 175349.
Withey, Z., Awan, A., Duguma, N., Fell, E., Martinez, N. J., Neary, E., … & Gweon, H. S. (2023). Mycobial community assemblages in sink drains across a university campus. Environmental DNA, 5(1), 212-224.
Prasedya, E. S., Kurniawan, N. S. H., Ardiana, N., Ilhami, B. T. K., Mulyaningsih, T., Astuti, S. P., … & Widyastuti, S. (2022). Effects of Fermented Seaweed Fertilizer Treatment on Paddy Amino Acid Content and Rhizosphere Microbiome Community. Fermentation, 8(9), 420.
Prasedya, E. S., Kurniawan, N. S. H., Kirana, I. A. P., Ardiana, N., Abidin, A. S., Ilhami, B. T. K., … & Nikmatullah, A. (2022). Seaweed Fertilizer Prepared by EM-Fermentation Increases Abundance of Beneficial Soil Microbiome in Paddy (Oryza sativa L.) during Vegetative Stage. Fermentation, 8(2), 46.
Del Álamo, A. C., Pariente, M. I., Molina, R., & Martínez, F. (2022). Advanced bio-oxidation of fungal mixed cultures immobilized on rotating biological contactors for the removal of pharmaceutical micropollutants in a real hospital wastewater. Journal of Hazardous Materials, 425, 128002.
Withey, Zoe, et al. “Characterization of communal sink drain communities of a university campus.” Environmental DNA (2021).
Семенов, М. В. (2019). Метабаркодинг и метагеномика в почвенно-экологических исследованиях: успехи, проблемы и возможности. Журнал общей биологии, 80(6), 403-417.
Kononenko, N., Baranova, E., Dilovarova, T., Akanov, E., & Fedoreyeva, L. (2020). Oxidative Damage to Various Root and Shoot Tissues of Durum and Soft Wheat Seedlings during Salinity. Agriculture, 10(3), 55.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.