HigherPurity™ Tissue DNA Extraction Kit (Reagent)
HigherPurity™ Tissue DNA Extraction Kit (Reagent) provides a fast, simple, and efficient reagent-based method for purifying high-quality genomic DNA from fresh or frozen animal tissues.
The optimized protocol — including lysis, protein removal, precipitation, washing, and hydration — ensures 5–500 µg of pure DNA, ready for PCR, cloning, or sequencing.
HigherPurity™ Tissue DNA Extraction Kit (Reagent) offers a highly efficient, rapid, and convenient solution for extracting genomic DNA from a variety of fresh or frozen solid tissues.
This reagent-based protocol provides a straightforward workflow that eliminates the need for columns or hazardous organic solvents.
The procedure includes tissue lysis, protein removal, DNA precipitation, washing, and hydration, resulting in pure genomic DNA suitable for all downstream molecular biology applications.
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Price: 91.20 € – 423.00 €
Detailed information:
Advantages & Features
- High efficiency: 5-100 µg of DNA from a 10 mg Animal Tissue Sample and 50-500 µg from a 100 mg.
- High purity: DNA ready-to-use in all molecular biology applications.
- Fast and simple: minimal handling and short processing time.
- Safe: avoids phenol/chloroform extraction and organic solvents.
- Reagent-based: ideal for labs preferring chemical extraction instead of column purification.
- Versatile: works with both fresh and frozen animal tissues.
Specifications
Includes
Includes for 20 rxn:
– 125 mL S2 Buffer
– 50 mL S3 Buffer
– 20 mg Proteinase K
– 0.65 mL RNAse A Solution (10 mg/mL)
– 50 mL EB Buffer
Download documentation
Datasheet MSDSApplications
Purified DNA suitable for all common Molecular Biology applications, such as:
- RT-PCR and PCR.
- Southern blotting.
- RFLP.
- Cloning.
- DNA sequencing.
- Genotyping and molecular diagnostics research (RUO).
Tables & Figures
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Quality Control
– Tested on a lot-to-lot basis by isolating Total DNA from 5 mg of Frozen Tissue.
– DNA purified is analysed by:
- Spectrophotometer: Ratio 260/ 280 (1.6-1.8)
- Agarose gel electrophoresis.
Advice
Storage, Shipping & Guarantee
- Shipped in: Ambient Temperature.
- Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC) and and RNAse A (-20 ºC). If any kit reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves, and allow to cool to RT before use.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
1 review for HigherPurity™ Tissue DNA Extraction Kit (Reagent)
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Aisha Rahman –
We applied this tissue DNA extraction reagent kit to process small biopsy samples in a research setting. The workflow is compatible with standard microcentrifugation equipment available in most molecular labs. Instructions are clearly written, which helps maintain consistency across different operators. The extracted DNA worked well for downstream PCR analysis in our experiments.