HigherPurity™ Yeast DNA Isolation Kit

Name: HigherPurity™ Yeast DNA Isolation Kit
SKU: AN0080
Availability: InStock

Efficient kit for high-quality genomic DNA purification from yeast.

The HigherPurity™ Yeast DNA Isolation Kit provides a fast, consistent, and efficient method for purifying genomic DNA from yeast and other fungal samples. It combines lyticase enzymatic digestion, glass bead disruption, and alkaline SDS lysis, followed by silica column binding to ensure inhibitor-free DNA recovery.

Purified DNA is suitable for PCR, sequencing, Southern blotting, and restriction enzyme digestion. The complete workflow takes under 90 minutes and consistently delivers high-quality DNA free of contaminants.

For filamentous fungi or macroscopic fungi, the yeast protocol may be used as a reference and may require optimization.

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Price: 160.90 € – 673.80 €

size

50 rxn

100 rxn

250 rxn

SKU: AN0080 Categories: DNA Extraction Kits, DNA & RNA Extraction

Detailed information:

Advantages & Features

Reliable: proven performance on yeast and other fungi.
Safe: no phenol/chloroform required.
High-quality DNA: ready-to-use for molecular biology.
Fast protocol: results in <90 min.
Spin-column technology ensures inhibitor removal.

Specifications

Specification	Description
Sample type	Yeast (50 mg)
Technology	Enzymatic + mechanical lysis + silica spin column
Protocol time	~88 minutes
Yield	High-quality genomic DNA
Purity	A260/A280 = 1.7–1.9
Formats	50 rxn, 100 rxn, 250 rxn
Includes	Buffers, Proteinase K, Lyticase, columns, tubes
Storage	RT (buffers), 4 ºC/–20 ºC (Proteinase K)
Intended use	Research Use Only

Includes

Includes for 50 rxn:
– 50 Minispin columns
– 50 collection tubes
– 35 mL BLL Buffer
– 20 mL BLY Buffer
– 15 mg Proteinase K
– 5,000 U Lyticase
– 30 mL WB1 Buffer
– 6 mL WB2 Buffer
– 10 mL EB Buffer

Download documentation

Datasheet

MSDS

Applications

PCR template preparation.
Restriction enzyme digestion.
Automated sequencing.
Southern blotting.

Tables & Figures

Quality Control

Tested lot-to-lot by isolating DNA from 50 mg yeast.
DNA quality confirmed by spectrophotometer (A260/A280 = 1.7–1.9).
Integrity checked by agarose gel electrophoresis.

Advice

Store Proteinase K at –20 ºC for long-term stability.
Ensure efficient homogenization with glass beads for optimal yields.

Storage, Shipping & Guarantee

Shipped in: Ambient Temperature.
Storage*: All components can be stored at Room Temperature, except RNAse A, Rupture Buffer and Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

Citations

FOROUGH, S., KUMARSS, A., AZAM, H., & MOHADDESEH, L. (2022). Application of Saccharomyces cerevisiae isolated from industrial effluent for zinc biosorption and zinc-enriched SCP production for human and animal. Food Science and Technology.
Petkova, Mariana, et al. “Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs.” Microorganisms 9.7 (2021): 1346.
Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.