PRImeZOL™ Reagent

– 2 x 50 mL PRImeZOL™ Reagent (ready-to-use)

• RNA extraction for RT-PCR, qPCR, RNA-Seq, and Northern blotting.

• DNA purification for PCR, genotyping, and sequencing.

• Protein isolation for Western blot and proteomics.

• Gene expression studies in mammalian, bacterial, yeast, and plant systems.

• Population genetics & epidemiology requiring multi-analyte recovery.

Individual recovery of DNA and proteins requires sequential phase separation and precipitation steps described in the datasheet.

Each batch is tested for:

• • RNA yield & integrity using total RNA extraction from cultured cells.

  • Absence of RNase/DNase contamination.

  • Consistency in phase separation and recovery.

• Always work under RNase-free conditions.

• Use appropriate safety equipment when handling phenol-based reagents.

• For best performance, process fresh or properly preserved samples.

• For complete removal of residual genomic DNA, optional DNase treatment after RNA isolation is recommended for highly sensitive applications.

• Shipped at: Ambient Temperature.
• Storage: Store at 2-8°C, protect from light. Do not freeze.

• Ábalos-Martínez, J., Visiedo, F., Cascajo-Almenara, M. V., Santos-Rosendo, C., Melero-Jiménez, V., Santos-Ocaña, C., … & Bugatto, F. (2026). Mitochondrial Dysfunction Combined with Elevated CoQ10 Levels Specifically in Placental Cytotrophoblasts Suggests a Role for Mitophagy in Preeclampsia. Biology, 15(2), 139.
• González-Correa, C., Miñano, S., Moleón, J., Toral, M., Robles-Vera, I., Sánchez, M., … & Duarte, J. (2025). The probiotic Limosilactobacillus fermentum CECT5716 enhances the antihypertensive response to hydrochlorothiazide in spontaneously hypertensive rats. Gut Microbes, 17(1), 2586324.
• Wardana, M. T. E., Oktarina, D. A. M., & Pramana, A. A. C. (2025). Toxicology Study and MMP-1 and COL1A1 Expression of Malang Green Apple Juice Fermented by Lactobacillus plantarum DAD-13 and Saccharomyces cerevisiae Fermipan on Human Fibroblast: Potential Modality of Anti-aging. F1000Research, 14, 1226.
• García, B., Conty, A., Fernández-Celis, A., Mouzo, D., Gil, C., Garmendia-Antoñana, N., … & Lasa, I. (2025). Dual transcriptomic profiling of Staphylococcus aureus endocarditis in a porcine model reveals strong parallels with human infection. mBio, e02316-25.
• Mohamed, A. E. E., & Soliman, M. A. Emotional Stress as A-Major Risk Factor for Type 1 Myocardial Infarction. The Egyptian Journal of Hospital Medicine (October 2025), 101, 5284-5291.
• Ramasamya, D., Ashaarib, N. S., Yusofc, Z. N. B., Wahabf, A. A., Zakariab, I. I., & Ismaila, S. RESEARCH ARTICLE In vitro antiviral activity of Coriandrum sativum L. seed crude extracts against respiratory syncytial virus.  AsPac J. Mol. Biol. Biotechnol Vol. 33 (3): 78-94
• Zhang, W., Li, L., Yang, G., Li, C., Deng, X., Huang, X., & Cheng, G. (2025). Investigating the Mechanism of β-Elemene-Mediated LINC00511 Modulation to Suppress Cisplatin Resistance in Lung Cancer. Journal of Visualized Experiments (JoVE), (222), e68825.
• Mora-Molina, R., Fernández-Farrán, F. J., López-Rivas, A., & Palacios, C. (2025). Activation of the integrated stress response and loss of cFLIPL under glutamine limitation induce IL-8 gene expression and secretion in glutamine-dependent tumor cells. Cell Death Discovery, 11(1), 1-14.
• Stoeva, S., Vankova, D., Sokrateva, T., & Nashar, M. (2025). The Potential of Sulfur-Containing Mineral Water and Sulfur-Containing Bioactive Compounds to Modulate Inflammatory Markers in Human Intestinal Epithelial Cells: A Comparative Study.
• Molina, R. M., El Yousfi, Y., Hagenlocher, C., Fernández-Farrán, F. J., Rehm, M., & Lopez-Rivas, A. (2025). REDD1/DDIT4 counteracts endoplasmic reticulum stress-induced apoptosis by controlling the expression of death receptor TRAILR2/DR5 in cancer cells.
• Sádaba, A., Garaikoetxea, M., Tiraplegui, C., San-Ildefonso-García, S., Goñi-Olóriz, M., Fernández-Celis, A., … & López-Andrés, N. (2025). The Presence of Adipose Tissue in Aortic Valves Influences Inflammation and Extracellular Matrix Composition in Chronic Aortic Regurgitation. International Journal of Molecular Sciences, 26(7), 3128.
• Vatov, E., & Gechev, T. (2025). Delay in flowering time in Col-0 under water deficit and in ddc triple knockout mutant. bioRxiv, 2025-03.
• El Yousfi, Y., Fernández-Farrán, F. J., Oliver, F. J., López-Rivas, A., & Yerbes, R. (2025). Regulation of ER stress-induced apoptotic and inflammatory responses via YAP/TAZ-mediated control of the TRAIL-R2/DR5 signaling pathway. Cell Death Discovery, 11(1), 1-12.
• Fournier, M., Javary, J., Roh, V., Fournier, N., & Radtke, F. (2024). Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer. EMBO Molecular Medicine, 1-34.
• Garrido Jimenez, Álvaro, et al. Estudios lipidómicos en macrófagos. Regulación de los niveles lipídicos por lipinas y su impacto en la biología del macrófago y en procesos inflamatorios. 2023.
• Pérez-Cabello JA, Silvera-Carrasco L, Franco JM, Capilla-González V, Armaos A, Gómez-Lima M, García-García R, Yap XW, Leal-Lasarte M, Lall D, Baloh RH, Martínez S, Miyata Y, Tartaglia GG, Sawarkar R, García-Domínguez M, Pozo D, Roodveldt C. MAPK/MAK/MRK overlapping kinase (MOK) controls microglial inflammatory/type-I IFN responses via Brd4 and is involved in ALS. Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2302143120. doi: 10.1073/pnas.2302143120. Epub 2023 Jul 3. PMID: 37399380; PMCID: PMC10334760.
• Lazarova, M.; Stefanova, M.; Denev, P.; Taseva, T.; Vassileva, V.; Tasheva, K. Neuroprotective Effect of Marrubium vulgare Extract in Scopolamine-Induced Cognitive Impairment in Rats: Behavioral and Biochemical Approaches. Biology 2024, 13, 426.
• Pérez-Cabello, J. A., Silvera-Carrasco, L.,  et al. (2023).  MAPK/MAK/MRK overlapping kinase (MOK) controls microglial inflammatory/type-I IFN responses via Brd4 and is involved in ALS pathophysiology.
• Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
• Pacchini, S., Piva, E., Schumann, S., Irato, P., Pellegrino, D., & Santovito, G. (2023). An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid. Antioxidants, 12(2), 352.
• Pacchini, S., Piva, E., Schumann, S., Irato, P., Pellegrino, D., & Santovito, G. (2023). Are Antarctic Fish Adapted to Face Global Changes? A Study on Antioxidant Enzymes Gene Expression in Trematomus newnesi from Ross Sea, Experimentally Exposed to PFOA.
• Yerbes, R., Mora-Molina, R., Fernández-Farrán, F. J., Hiraldo, L., López-Rivas, A., & Palacios, C. (2022). Limiting glutamine utilization activates a GCN2/TRAIL-R2/Caspase-8 apoptotic pathway in glutamine-addicted tumor cells. Cell Death & Disease, 13(10), 906.
• Bakiu, R., Piva, E., Schumann, S., Irato, P., & Santovito, G. (2022). Metallothionein Expression as Physiological Response Against Metal Toxicity in the Striped Rockcod Trematomus hansoni.
• Pizzino, F., Furini, G., Casieri, V., Mariani, M., Bianchi, G., Storti, S., … & Lionetti, V. Plasma exosome microRNA-21-5p depicts magnitude of late reverse ventricular remodeling after surgical repair of primary mitral valve regurgitation. Frontiers in Cardiovascular Medicine, 1984.
• Piva, E., Schumann, S., Dotteschini, S., Brocca, G., Radaelli, G., Marion, A., … & Santovito, G. (2022). Antioxidant Responses Induced by PFAS Exposure in Freshwater Fish in the Veneto Region.
• Mora-Molina, R., Stöhr, D., Rehm, M., & López-Rivas, A. (2022). cFLIP downregulation is an early event required for endoplasmic reticulum stress-induced apoptosis in tumor cells. Cell Death & Disease, 13(2), 1-12.
• Reverte, Marta, et al. “The antioxidant response favors Leishmania parasites survival, limits inflammation and reprograms the host cell metabolism.” PLoS pathogens 17.3 (2021): e1009422.
• Gómez-Melero, S., García-Maceira, F. I., García-Maceira, T., Luna-Guerrero, V., Montero-Peñalvo, G., Túnez-Fiñana, I., & Paz-Rojas, E. (2021). Amino Terminal Recognition by a CCR6 Chemokine Receptor Antibody Blocks CCL20 Signaling and IL-17 Expression via β-arrestin.
• García-Maceira, T., García-Maceira, F. I., González-Reyes, J. A., & Paz-Rojas, E. (2020). Highly enhanced ELISA sensitivity using acetylated chitosan surfaces.BMC Biotechnology, 20(1), 41.
• Caballero, L. F. (2018). Estudio de las desaturasas de ácidos grasos FADS1 y FADS2 en macrófagos.
• Robledo Mahón, T. (2018). Estudio de los procesos biológicos y de la estructura de las comunidades microbianas en el proceso de compostaje de lodos de depuradora de aguas residuales urbanas, en sistemas de membranas semipermea.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.

Toxic if swallowed or in contact with skin; causes burns. Work in fume hood with appropriate PPE.

For more info, please check its Material Safety Data Sheet available in this website.

Q: Can PrimeZOL™ Reagent isolate RNA, DNA, and protein simultaneously?
A: Yes. Following the recommended protocol, RNA is recovered from the aqueous phase, and DNA and proteins can be sequentially precipitated from the interphase and organic phase.

Q: Is PrimeZOL™ compatible with small RNA (miRNA)?
A: Yes, small RNAs are efficiently recovered without additional modifications.

Q: How does PrimeZOL™ compare to Product T?
A: PrimeZOL™ provides equivalent yield and purity at a more cost-effective price.