HigherPurity™ Blood/Cultured Cell Total RNA Kit

Includes for 50 rxn:
– 120 mL RL Buffer
– 25 mL Buffer
– 30 mL Wash Buffer 1
– 15 mL Wash Buffer 2 (concentrate)
– 10 mL RNase-free Water
– 50 RNAprep spin column
– 50 Filter Column
– 100 Collection Tube
– 50 Elution Tube

• RT-PCR.
• Northern Blotting.
• Primer Extension.
• mRNA Selection.
• cDNA synthesis and cloning.
• Gene expression analysis.

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• Total RNA is isolated from 300 μl of fresh whole Human blood.
• Purified RNA is quantified using a spectrophotometer with a typical yield of more than 2 μg of Total RNA and an A260/A280 ratio of 1.8-2.0.
• Quality is further checked by agarose gel electrophoresis.

• Shipped in: Ambient Temperature.
• Storage: All components can be stored at Room Temperature (15 – 25 °C).

• Κορμέντζα, Ελισάβετ. “Μεταβολή της βιολογικής συμπεριφοράς των καρκινικών κυττάρων 22Rv1, πριν και μετά την εγκατάσταση αλλομοσχεύματος σε ανοσοανεπαρκή θήλεα και άρρενα ποντίκια SCID.“ (2021).
• Παπαδάκη, Ε. (2021). Σύγκριση της βιολογικής συμπεριφοράς των καρκινικών ορμονοευαίσθητων κυττάρων προστάτη 22Rv1, πριν και μετά την εγκατάσταση αλλομοσχεύματος σε ανοσοανεπαρκείς μύες.
• Μωυσέως, Ά. (2021). Σύγκριση της βιολογικής συμπεριφοράς των καρκινικών ορμονοάντοχων κυττάρων προστάτη DU145, πρίν και μετά την εγκατάσταση αλλομοσχεύματος σε SCID-mice.
• Giatromanolaki, A., Fasoulaki, V., Kalamida, D., Mitrakas, A., Kakouratos, C., Lialiaris, T., & Koukourakis, M. I. (2019). CYP17A1 and Androgen-Receptor Expression in Prostate Carcinoma Tissues and Cancer Cell Lines. Current Urology, 13(3), 157-165.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Blood RNA extraction kit, Cell culture RNA isolation kit, Total RNA purification blood cells, RNA extraction cultured cells, RNA isolation blood samples

Also known as:

• Spanish: Kit de extracción de ARN de sangre y células cultivadas, Aislamiento de ARN total, Purificación de ARN celular
• French: Kit d’extraction ARN sang et cellules cultivées, Isolation ARN total, Purification ARN cellulaire
• German: RNA-Extraktionskit für Blut und Zellkultur, Gesamt-RNA-Isolation, RNA-Reinigung Zellen
• Italian: Kit estrazione RNA sangue e cellule coltivate, Isolamento RNA totale, Purificazione RNA cellulare

Q: What types of samples can be processed with this kit?
A: The kit is designed for total RNA purification from cultured cells and whole blood samples.

Q: What is the typical RNA yield obtained with this kit?
A: Typical yields range from 2 to 30 µg of total RNA, depending on the sample type and input amount.

Q: What is the expected purity of the extracted RNA?
A: The RNA typically shows A260/A280 ratios between 1.8 and 2.0, suitable for downstream applications.

Q: What downstream applications is the extracted RNA suitable for?
A: The purified RNA is suitable for RT-PCR, cDNA synthesis, Northern blotting, and related applications.