HigherPurity™ Plant RNA Purification Kit

Includes for 50 rxn:
– 50 RNAprep spin columns
– 50 Collection tubes (2 mL)
– 50 Filter column
– 50 Elution tubes
– 30 mL L1 Buffer
– 30 mL L2 Buffer
– 30 mL WB1 Buffer
– 20 mL WB2 Buffer
– 6 mL RNase- free water

• Gene expression analysis in plants.

• Functional genomics.

• Plant stress and defense studies.

• Transcriptomics and Northern blotting.

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• RNA isolated from 100 mg young leaves.

• Typical yield: >10 µg RNA with A260/A280 = 1.9–2.1.

• RNA integrity verified by agarose gel electrophoresis.

• Grind plant tissue in liquid nitrogen before starting.

• Use RNase-free consumables to avoid degradation.

• Shipped in: Ambient Temperature.
• Storage: All components can be stored at Room Temperature (15 – 25 °C), except RNAse A (-20 °C).

• Lukacova, Zuzana, et al. “Silicon and cadmium interaction of maize (Zea mays L.) plants cultivated in vitro.” Biologia (2021): 1-13.
• Paredes‐Páliz, K., Rodríguez‐Vázquez, R., Duarte, B., Caviedes, M. A., Mateos‐Naranjo, E., Redondo‐Gómez, S., … & Pajuelo, E. (2018). Investigating the mechanisms underlying phytoprotection by plant growth‐promoting rhizobacteria in Spartina densiflora under metal stress. Plant Biology, 20(3), 497-506.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q: What sample size is recommended?
A: 100 mg of young leaves typically yields up to 30 µg RNA.

Q: Is phenol required?
A: No, the kit avoids phenol–chloroform extraction.

Q: Can RNA be used for qPCR?
A: Yes, RNA is ready-to-use for RT-PCR, qPCR, and Northern blotting.