HigherPurity™ Total RNA Extraction Kit

Includes for 50 rxn:
– 20 mL RNA Buffer Lysis
– 27 mL RNA Wash Buffer 1
– 16 mL RNA Wash Buffer 2
– 5 mL RNase-free ddH2O
– 50 RNAprep spin column
– 50 Filter Column
– 100 Collection tube (2 mL)
– 50 microtube (1.5 mL)
– 50 Micropestle

• RT-PCR and qPCR.

• Northern blotting.

• Primer extension assays.

• microRNA cloning and expression arrays.

• RNA cleanup for transcriptomics.

• Each batch tested for RNA yield, purity (A260/A280 ratio), and integrity.

• Validated for multiple sample types.

• Use micropestles to homogenize tissue samples thoroughly.

• Always process samples quickly to preserve RNA quality.

• Shipped in: Ambient Temperature.
• Storage: Room Temperature (15–25 °C).

• Zhou, C., & Liu, S. (2021). Evaluation of the efficacy of MST-312, as a telomerase inhibitor, in the treatment of patients with multiple myeloma after stem cell transplantation. Cellular and Molecular Biology, 67(4), 115-120.
• Φορτώση, Αλεξάνδρα Ουρανία. “Συγκριτική μελέτη της μεταβολής της βιολογικής συμπεριφοράς και του φαινοτύπου της καρκινικής κυτταρικής σειράς πνεύμονα Α549, μετά από ανάπτυξη αντοχής στην ακτινοβολία.” (2021).
• Ortega-Pinazo, J., Serrano-Castro, P. J., Vida-Botella, M., Martínez, B., Pinto-Medel, M. J., Oliver-Martos, B., … & Victoria, U. G. C. (2021). Comparing RNA extraction methods to face the variations in RNA quality using two human biological matrices.
• Wei, Cen, et al. “LncRNA-IUR Sponges miR-24 to Upregulate P53 in Laryngeal Squamous Cell Carcinoma.” Cancer Management and Research 12 (2020): 11639-11647.
• Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.
• Ke, J., Shen, Z., Hu, W., Li, M., Shi, Y., Xie, Z., & Wu, D. (2020). LncRNA DCST1-AS1 Was Upregulated in Endometrial Carcinoma and May Sponge miR-92a-3p to Upregulate Notch1. Cancer Management and Research, 12, 1221.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q: What is the maximum yield?
A: Up to 60 µg RNA, depending on sample type.

Q: What types of samples can be processed?
A: Cells, tissues, bacteria, yeast, fungi, and paraffin-fixed samples.

Q: Does it require phenol/chloroform?
A: No, the protocol is phenol-free.