6X B/Loading Buffer
Ready-to-use DNA loading dye for convenient agarose gel electrophoresis and sample tracking.
6× B Loading Buffer from Canvax™ is a ready-to-use DNA loading dye formulated to simplify agarose gel electrophoresis. It combines density agents and tracking dyes to ensure reliable migration control and rapid sample visualization.
This buffer allows convenient loading of DNA or PCR products directly onto agarose gels, enabling accurate monitoring of electrophoretic separation without interfering with downstream applications such as cloning or gel extraction. Its 6× concentration provides flexibility and easy dilution for routine molecular biology workflows.
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Price: 31.00 €
Detailed information:
Advantages & Features
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Ready-to-use formulation: No preparation required; simply mix with your DNA sample.
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Dual tracking dyes: Contains Bromophenol Blue and Xylene Cyanol FF for clear migration visualization.
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High density: Facilitates easy sample sinking into gel wells.
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Compatible with standard buffers: Works with both TAE and TBE electrophoresis systems.
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Non-interfering: Does not affect DNA recovery or downstream enzymatic reactions.
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Long shelf-life: Stable at room temperature for extended storage.
Specifications
- Concentration: 0.03% bromophenol blue, 60% Glycerol, 10 mM Tris-HCl (pH 7.6) and 60 mM EDTA.
Includes
– 5 x 1 mL of 6x Loading Buffer
Download documentation
Datasheet MSDSApplications
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DNA gel electrophoresis.
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Visualization of sample migration fronts.
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PCR product analysis.
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Restriction fragment size estimation.
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Gel loading standardization.
Tables & Figures
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Quality Control
- Each lot is tested for consistent migration behavior, absence of nuclease activity, and optical clarity.
Advice
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Mix 1 volume of 6× B Loading Buffer with 5 volumes of DNA sample.
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Avoid over-loading the wells to maintain sharp bands.
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For accurate size comparison, run a DNA ladder alongside samples.
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Store tightly closed to prevent evaporation.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: 4 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What dyes are included in the 6× B Loading Buffer?
It contains Bromophenol Blue and Xylene Cyanol FF, which migrate at approximately 300 bp and 4 kb respectively in 1 % agarose gels.
Q2: Can this buffer be used with RNA samples?
Yes, it is suitable for both DNA and RNA electrophoresis when non-denaturing conditions are applied.
Q3: Does it interfere with gel extraction kits?
No, the dyes and components do not affect DNA recovery using standard purification kits.
