Bst DNA Polymerase

Unit definition:
One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65 C in 20 mMTris-HCl pH 8.8, 10 mM (NH₄)₂SO₄,10 mM KCl, 2 mM MgSO₄, 0.1% Triton X-100, 30 nM M13mp18 ssDNA, 70 nMM13 sequencing primer(-47) 24-mer 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [³³P]dCTP) and 0.1 mg/mL BSA.

– Bst DNA Polymerase (8 U/μL)
– Reaction Buffer (10x)
– 100mM MgSO₄ solution

• Isothermal DNA amplification (LAMP).

• DNA labeling and probe generation.

• Primer extension assays.

• Rolling circle amplification (RCA).

• Genotyping and molecular diagnostics.

Some uses for this product may require licenses. Canvax does not encourage or support the unauthorized or unlicensed use of patented nucleic acid amplification processes for isothermal amplification, whole genome amplification (WGA), multiple displacement amplification (MDA), and Next Generation sequencing. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.

• Purity: >99% by SDS PAGE.
• Each batch is tested for specific activity, fidelity, and absence of DNase, RNase, and endonuclease contamination to ensure high performance and reproducibility.
• 10 µL of the enzyme was tested for E. coli genomic DNA contamination by PCR amplifying with the E.coli 16S ribosomal primers.

• Perform reactions at 60–65 °C for optimal activity.

• Use high-quality DNA templates for best results.

• Avoid repeated freeze–thaw cycles to maintain enzyme stability.

• Shipped in: Gel pack.
• Storage: -20 °C (NON Frost-Free Freezer).

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What is the optimal temperature for amplification?
The enzyme performs best at 60–65 °C, depending on the assay design.

Q2: Does Bst DNA Polymerase have 5’→3′ exonuclease activity?
No. It lacks 5’→3′ exonuclease activity but has strong strand-displacement activity.

Q3: Can it be used for LAMP reactions?
Yes. It is the preferred enzyme for loop-mediated isothermal amplification workflows.