Horse-Power™ GC PCR Enhancer Kit

Name: Horse-Power™ GC PCR Enhancer Kit
SKU: P0080
Price: 42.12 EUR
Availability: InStock

Enhancer kit designed to improve PCR amplification of GC-rich DNA templates.

Horse Power™ GC PCR Enhancer Kit is formulated to improve PCR amplification of GC-rich templates. It enhances yield, specificity, and fidelity in difficult DNA regions.

The kit provides ready-to-use reagents that simplify PCR setup and enable consistent amplification across a wide GC content range.

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100 rxn

Price: 42.12 €

SKU: P0080 Categories: End Point PCR, DNA Polymerases

Detailed information:

Advantages & Features

Enhanced amplification: increases yield and specificity in GC-rich templates.
Improved fidelity: supports accurate amplification of difficult regions.
Broad compatibility: works with most DNA polymerases and PCR systems.
Ready-to-use: simplifies setup without additional optimization steps.
Reproducible results: ensures consistent performance in complex assays.

Specifications

Assay Conditions: 25mM Tris-HCl pH9,0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0,1 mg/mL gelatine, 200 μM de dATP, dGTP, dTTP, 100μM[α32-P]dCTP (0,05μCi/nmol) and 12,5 μg activated salmon sperm DNA.
Unit Definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes

Includes:
– Horse-Power™ Taq Polymerase (5 U/μL)
– PCR Buffer (10x)
– 25 mM MgCl₂ (1.5 mL)
– TruePure™ dNTPs (2 mM each)
– GC Enhancer Solution (10x)

Download documentation

Datasheet

MSDS

Applications

Amplification of GC-rich DNA regions.
Long-fragment PCR optimization.
High-fidelity PCR assays.
Cloning and sequencing of difficult targets.

Tables & Figures

Quality Control

Each batch is tested for amplification performance, reproducibility, and enzyme compatibility.
Undetected bacterial DNA (by PCR).
Undetectable nucleases activity (endo-, exo, and ribonucleases).

Advice

Add enhancer to your PCR mix according to recommended ratios.
Optimize Mg²⁺ concentration for best performance.
Avoid repeated freeze–thaw cycles; store aliquots when possible.

Storage, Shipping & Guarantee

Shipped in: Gel Pack.
Storage: -20 ºC (NON Frost-Free Freezer).

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in thi

Customers Review

FAQs

Q1: What makes Horse-Power™ GC PCR Enhancer effective on GC-rich DNA?
It contains proprietary additives that destabilize secondary structures and enhance denaturation.

Q2: Is it compatible with Taq and high-fidelity polymerases?
Yes, it works with a wide range of DNA polymerases and PCR master mixes.

Q3: Does it affect downstream sequencing or cloning?
No, it does not interfere with subsequent enzymatic reactions or DNA manipulations.