Horse-Power™ Taq DNA Polymerase with dNTPs

Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl₂, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

– 100 μL Horse-Power™ Taq Polymerase (5 U/μL)
– 25 mM MgCl₂ (1.5 mL)
– 1.5 mL Buffer (10x)
– 1 mL TruePure™ Ultrapure dNTPs (2 mM each)

• Routine PCR.

• Genotyping and colony screening.

• DNA fragment amplification.

• Cloning and sequencing template generation.

• High-throughput PCR workflows.

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• Each batch is tested for purity, activity, specificity, and absence of DNase/RNase contamination, ensuring reproducible amplification results.

• Use 1–2.5 U per 50 µL PCR reaction.

• Thaw reagents on ice and mix gently before use.

• For GC-rich templates, optimize annealing temperature or Mg²⁺ concentration.

• Shipped in: Gel pack.
• Storage: -20 °C (NON Frost-Free Freezer).

• Hristova, P. M. (2025). Distribution of Virulence Factors in Vancomycin-Resistant Enterococci Isolated from Clinical and Intestinal Samples. Microorganisms, 14(1), 90.
• RUSENOVA, N., KRUSTEV, S., ATANASOV, A., RUSENOV, A., & STANILOVA, S. (2020) Differentiation of Staphylococcus pseudintermedius in the Staphylococcus intermedius Group (SIG) by Conventional and Molecular Methods. thought, 10, 11.
• Fagín, E., Bravo, I., Garrido, J. L., Rodríguez, F., & Figueroa, R. I. (2019) Scrippsiella acuminata versus Scrippsiella ramonii: A Physiological Comparison. Cytometry Part A.
• Robledo-Mahón, T., Silva-Castro, G. A., Kuhar, U., Jamnikar-Ciglenečki, U., Barlič-Maganja, D., Aranda, E., & Calvo, C. (2019). Effect of Composting Under Semipermeable Film on the Sewage Sludge Virome. Microbial ecology, 1-9.
• Negreiros, A. M. P., Sales Júnior, R., León, M., Melo, N. J. D. A., Michereff, S. J., Ambrósio, M. M. D. Q., … & Armengol, J. Identification and pathogenicity of Macrophomina species collected from weeds in melon fields in Northeastern Brazil. Journal of Phytopathology.
• Rêgo, T. J. S., Elena, G., Correia, K. C., Tovar‐Pedraza, J. M., Câmara, M. P. S., Armengol, J., … & Berbegal, M. Genetic diversity and population structure of Lasiodiplodia theobromae from different hosts in northeastern Brazil and Mexico. Plant Pathology.
• Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal, 325, 257-269.
• Ruiz-Estévez, M., Bakkali, M., Martín-Blázquez, R., & Garrido-Ramos, M. A. (2017). Identification and Characterization of TALE Homeobox Genes in the Endangered Fern Vandenboschia speciosa. Genes, 8(10), 275.
• Ruiz-Estévez, M., Bakkali, M., Martín-Blázquez, R., & Garrido-Ramos, M. A. (2017). Differential expression patterns of MIKCC-type MADS-box genes in the endangered fern Vandenboschia speciosa. Plant Gene, 12, 50-56.
• Navarro-Domínguez, B., Ruiz-Ruano, F. J., Camacho, J. P. M., Cabrero, J., & López-León, M. D. (2017). Transcription of a B chromosome CAP-G pseudogene does not influence normal Condensin Complex genes in a grasshopper. Scientific reports, 7(1), 17650.
• Núñez Delegido, E. (2017). Caracterización funcional de los genes CRD de Arabidopsis thaliana.
• Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal.
• Anaya Calvo-Rubio, G. (2017). Desarrollo y validación de un sistema diagnóstico rápido para la detección de alteraciones cromosómicas que provocan infertilidad en caballos basado en herramientas moleculares.
• Calvo‐Polanco, M., Sánchez‐Castro, I., Cantos, M., García, J. L., Azcón, R., Ruiz‐Lozano, J. M., … & Aroca, R. (2016). Effects of different arbuscular mycorrhizal fungal backgrounds and soils on olive plants growth and water relation properties under well‐watered and drought conditions. Plant, Cell & Environment, 39(11), 2498-2514.
• Núñez Delegido, E. (2017). Caracterización funcional de los genes CRD de Arabidopsis thaliana.
• León-Ortega, M., & González-Wangüemert, M. (2015). Characterization of 10 new tetranucleotide microsatellite markers for the European eagle owl, Bubo bubo: Useful tools for conservation strategies. Biochemical Systematics and Ecology, 63, 109-112.
• Demyda-Peyrás, S., Anaya, G., Bugno-Poniewierska, M., Pawlina, K., Membrillo, A., Valera, M., & Moreno-Millán, M. (2014). The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism. Theriogenology, 81(8), 1116-1122.
• Navarro-Domínguez, B., Cabrero, J., Camacho, J. P. M., & López-León, M. D. (2016). B-chromosome effects on Hsp70 gene expression does not occur at transcriptional level in the grasshopper Eyprepocnemis plorans. Molecular Genetics and Genomics, 291(5), 1909-1917.
• ALMENDROS, V. M. T. (2016). Identificación de especies de Dactylonectria e Ilyonectria asociadas al pie negro de la vid en Castilla la Mancha.
• Martínez-Miguel, P., Valdivielso, J. M., Medrano-Andrés, D., Román-García, P., Cano-Peñalver, J. L., Rodríguez-Puyol, M., … & López-Ongil, S. (2014). The active form of vitamin D, calcitriol, induces a complex dual upregulation of endothelin and nitric oxide in cultured endothelial cells. American Journal of Physiology-Endocrinology and Metabolism, 307(12), E1085-E1096.
• Daddaoua, A., Molina-Santiago, C., de la Torre, J., Krell, T., & Ramos, J. L. (2014). GtrS and GltR form a two-component system: the central role of 2-ketogluconate in the expression of exotoxin A and glucose catabolic enzymes in Pseudomonas aeruginosa. Nucleic acids research, 42(12), 7654-7665.
• Nieves-Morión, M., Lechno-Yossef, S., López-Igual, R., Frías, J. E., Mariscal, V., Nürnberg, D. J., … & Flores, E. (2017). Specific glucoside transporters influence septal structure and function in the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. Journal of Bacteriology, JB-00876.
• Sierra, A. M. P., Sala, B. M., Santana, M. L., Jiménez, J. G., & Campos, P. A. (2012). Enfermedades causadas por en viveros de plantas ornamentales. In Boletin de Sanidad Vegetal: Plagas (Vol. 38, No. 1, pp. 143-156). Ministerio de Agricultura, Pesca y Alimentacion, Gobierno de España.
• Pérez-Sierra, A., Landeras, E., León, M., Berbegal, M., García-Jiménez, J., & Armengol, J. (2007). Characterization of Fusarium circinatum from Pinus spp. in northern Spain. Mycological research, 111(7), 832-839.
• Valverde-Tercedor, C., Montalbán-López, M., Perez-Gonzalez, T., Sanchez-Quesada, M. S., Prozorov, T., Pineda-Molina, E., … & Jimenez-Lopez, C. (2015). Size control of in vitro synthesized magnetite crystals by the MamC protein of Magnetococcus marinus strain MC-1. Applied microbiology and biotechnology, 99(12), 5109-5121.
• Mohammadi, H., Banihashemi, Z., Gramaje, D., & Armengol, J. (2012). Fungal pathogens associated with grapevine trunk diseases in Iran. Journal of Agricultural Science and Technology, 15(1), 137-150.
• Hernández-Hernández, J., Espino, A., Rodríguez-Rodríguez, J. M., Pérez-Sierra, A., León, M., Abad-Campos, P., & Armengol, J. (2010). Survey of diseases caused by Fusarium spp. on palm trees in the Canary Islands. Phytopathologia Mediterranea, 49(1), 84-88.
• Boughalleb, N., Salem, I. B., Beltrán, R., Vicent, A., Sierra, A. P., Abad‐Campos, P., … & Armengol, J. (2010). Occurrence of Monosporascus cannonballus in watermelon fields in Tunisia and factors associated with ascospore density in soil. Journal of phytopathology, 158(3), 137-142.
• Soto, E. C., Yáñez-Ruiz, D. R., Cantalapiedra-Hijar, G., Vivas, A., & Molina-Alcaide, E. (2012). Changes in ruminal microbiota due to rumen content processing and incubation in single-flow continuous-culture fermenters. Animal production science, 52(9), 813-822.
• Jroundi, F., Fernández-Vivas, A., Rodriguez-Navarro, C., Bedmar, E. J., & González-Muñoz, M. T. (2010). Bioconservation of deteriorated monumental calcarenite stone and identification of bacteria with carbonatogenic activity. Microbial ecology, 60(1), 39-54.
• Macias, M. I., Grande, J., Moreno, A., Domínguez, I., Bornstein, R., & Flores, A. I. (2010). Isolation and characterization of true mesenchymal stem cells derived from human term decidua capable of multilineage differentiation into all 3 embryonic layers. American journal of obstetrics and gynecology, 203(5), 495-e9.
• Clemente, A., Marín-Manzano, M. C., Jiménez, E., Arques, M. C., & Domoney, C. (2012). The anti-proliferative effect of TI1B, a major Bowman–Birk isoinhibitor from pea (Pisum sativum L.), on HT29 colon cancer cells is mediated through protease inhibition. British Journal of Nutrition, 108(S1), S135-S144.
• Alvarez, L. A., León, M., Abad-Campos, P., García-Jiménez, J., & Vicent, A. (2011). Genetic variation and host specificity of Phytophthora citrophthora isolates causing branch cankers in Clementine trees in Spain. European journal of plant pathology, 129(1), 103-117.
• Pérez-Sierra, A., León, M., Álvarez, L. A., Alaniz, S., Berbegal, M., García-Jiménez, J., & Abad-Campos, P. (2010). Outbreak of a new Phytophthora sp. associated with severe decline of almond trees in eastern Spain. Plant Disease, 94(5), 534-541.
• Jiménez-Hidalgo, M., Kurz, C. L., Pedrajas, J. R., Naranjo-Galindo, F. J., González-Barrios, M., Cabello, J., … & Fierro-González, J. C. (2014). Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin. Free Radical Biology and Medicine, 68, 205-219.
• Berbegal, M., Pérez-Sierra, A., Armengol, J., & Grünwald, N. J. (2013). Evidence for multiple introductions and clonality in Spanish populations of Fusarium circinatum. Phytopathology, 103(8), 851-861.
• Alaniz, S., Armengol, J., León, M., García-Jiménez, J., & Abad-Campos, P. (2009). Analysis of genetic and virulence diversity of Cylindrocarpon liriodendri and C. macrodidymum associated with black foot disease of grapevine. Mycological research, 113(1), 16-23.
• Mohammadi, H., Alaniz, S., Banihashemi, Z., & Armengol, J. (2009). Characterization of Cylindrocarpon liriodendri associated with black foot disease of grapevine in Iran. Journal of Phytopathology, 157(10), 642-645.
• Rafael, R., Márquez, M. C., Papke, R. T., & Ventosa, A. (2012). Multilocus sequence analysis of the family Halomonadaceae. International journal of systematic and evolutionary microbiology, 62(3), 520-538.
• Ruiz, R., Barroso-Deljesus, A., Lara, L., & Rubio, L. A. (2015). The use of multiple restriction enzymes in terminal restriction fragment length polymorphism analysis and identification of performance-related caecal bacterial groups in growing broiler chickens. The Journal of Agricultural Science, 153(08), 1491-1505.
• Dorado, J., Anaya, G., Bugno‐Poniewierska, M., Molina, A., Mendez‐Sanchez, A., Ortiz, I., … & Demyda‐Peyrás, S. (2016). First case of sterility associated with sex chromosomal abnormalities in a jenny. Reproduction in Domestic Animals.
• García-Carneros, A. B., García-Ruiz, R., & Molinero-Ruiz, L. (2014). Genetic and Molecular Approach to Verticillium dahliae Infecting Sunflower. Helia, 37(61), 205-214.
• Ortiz-Bustos, C. M., Testi, L., García-Carneros, A. B., & Molinero-Ruiz, L. (2016). Geographic distribution and aggressiveness of Harpophora maydis in the Iberian peninsula, and thermal detection of maize late wilt. European Journal of Plant Pathology, 144(2), 383-397.
• Soto, E. C., Molina-Alcaide, E., Khelil, H., & Yáñez-Ruiz, D. R. (2013). Ruminal microbiota developing in different in vitro simulation systems inoculated with goats’ rumen liquor. Animal Feed Science and Technology, 185(1), 9-18.
• Rodríguez-Ruano, S. M., Martín-Vivaldi, M., Martín-Platero, A. M., López-López, J. P., Peralta-Sánchez, J. M., Ruiz-Rodríguez, M., … & Martínez-Bueno, M. (2015). The Hoopoe’s uropygial gland hosts a bacterial community influenced by the living conditions of the bird. PloS one, 10(10), e0139734.
• Armengol, J., Vicent, A., León, M., Berbegal, M., Abad‐Campos, P., & García‐Jiménez, J. (2010). Analysis of population structure of Rosellinia necatrix on Cyperus esculentus by mycelial compatibility and inter‐simple sequence repeats (ISSR). Plant pathology, 59(1), 179-185.
• Pérez‐Sierra, A., López‐García, C., León, M., García‐Jiménez, J., Abad‐Campos, P., & Jung, T. (2013). Previously unrecorded low‐temperature Phytophthora species associated with Quercus decline in a Mediterranean forest in eastern Spain. Forest Pathology, 43(4), 331-339.
• González‐Domínguez, E., León, M., Armengol, J., & Berbegal, M. (2015). A Nested Polymerase Chain Reaction Protocol for in planta Detection of Fusicladium eriobotryae, Causal Agent of Loquat Scab. Journal of Phytopathology, 163(5), 415-418.
• Tello, M. L., Gramaje, D., Gómez, A., Abad-Campos, P., & Armengol, J. (2010). Analysis of phenotypic and molecular diversity of Phaeomoniella chlamydospora isolates in Spain. Journal of Plant Pathology, 195-203.
• Salem, I. B., Correia, K. C., Boughalleb, N., Michereff, S. J., León, M., Abad-Campos, P., … & Armengol, J. (2013). Monosporascus eutypoides, a cause of root rot and vine decline in Tunisia, and evidence that M. cannonballus and M. eutypoides are distinct species. Plant disease, 97(6), 737-743.
• González, M., Pérez‐Sierra, A., Serrano, M. S., & Sánchez, M. E. (2016). Two Phytophthora species causing decline of wild olive (Olea europaea subsp. europaea var. sylvestris). Plant Pathology.
• Fernández-Bedmar, Z., & Alonso-Moraga, A. (2016). In vivo and in vitro evaluation for nutraceutical purposes of capsaicin, capsanthin, lutein and four pepper varieties. Food and Chemical Toxicology, 98, 89-99.
• Ruiz-Ruano, F. J. (2014). Juan Pedro M. Camacho, Josefa Cabrero, Maria Dolores López-León, Diogo C. Cabral-de-Mello. Protocols for Cytogenetic Mapping of Arthropod Genomes, 381.
• Pacheco Piñol, S., & Roig Navarro, I. (2015). The role of ATR kinase during mammalian gametogenesis.
• Marcet Ortega, M., & Roig, I. (2016). Surveillance mechanisms in mammalian meiosis.
• CRESPO, J. A. (2016). Efecto de los tratamientos por termoterapia con agua caliente sobre la micoflora presente en plantas de vid injertadas.
• Agustí-Brisach, C., Cabral, A., González-Domínguez, E., Pérez-Sierra, A., León, M., Abad-Campos, P., … & Armengol, J. (2016). Characterization of Cylindrodendrum, Dactylonectria and Ilyonectria isolates associated with loquat decline in Spain, with description of Cylindrodendrum alicantinum sp. nov. European Journal of Plant Pathology, 145(1), 103-118.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What is the advantage of using TruePure™ dNTPs?
They provide ultrapure, nuclease-free nucleotides that improve reproducibility and amplification accuracy.

Q2: What fragment length can this enzyme amplify?
Typically up to 5 kb for standard templates.

Q3: Is it suitable for high-throughput PCR?
Yes. The formulation ensures batch-to-batch consistency for large-scale workflows.