Horse-Power™ Taq DNA Polymerase

Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl₂, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

 Includes for 500 U:
– 100 μL Horse-Power™ Taq Polymerase (5 U/μL)
– 25 mM MgCl₂ (1.5 mL)
– 1.5 mL Buffer (10x)

• Routine amplifications.
• Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
• Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
• PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).

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• Functionally tested in PCR.
• Undetected bacterial DNA (by PCR).
• Undetectable nucleases (endo-, exo, and ribonucleases) activities guaranteed by appropriate quality tests.

• Shipped in: Gel pack.
• Storage: -20 ºC (NON Frost-Free Freezer).

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• Mora-Sala, B., Cabral, A., León, M., Agustí-Brisach, C., Armengol, J., & Abad-Campos, P. Survey, Identification and Characterization of cylindrocarpon-like asexual morphs in Spanish forest nurseries. Plant Disease, (ja).
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• García-Carneros, A. B., García-Ruiz, R., & Molinero-Ruiz, L. (2014). Genetic and Molecular Approach to Verticillium dahliae Infecting Sunflower. Helia, 37(61), 205-214.
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• González‐Domínguez, E., León, M., Armengol, J., & Berbegal, M. (2015). A Nested Polymerase Chain Reaction Protocol for in planta Detection of Fusicladium eriobotryae, Causal Agent of Loquat Scab. Journal of Phytopathology, 163(5), 415-418.
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• CRESPO, J. A. (2016). Efecto de los tratamientos por termoterapia con agua caliente sobre la micoflora presente en plantas de vid injertadas.
• Agustí-Brisach, C., Cabral, A., González-Domínguez, E., Pérez-Sierra, A., León, M., Abad-Campos, P., … & Armengol, J. (2016). Characterization of Cylindrodendrum, Dactylonectria and Ilyonectria isolates associated with loquat decline in Spain, with description of Cylindrodendrum alicantinum sp. nov. European Journal of Plant Pathology, 145(1), 103-118.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Taq DNA polymerase, Thermostable DNA polymerase, PCR polymerase Taq, Recombinant Taq polymerase, DNA amplification enzyme

Also known as:

• Spanish: ADN polimerasa Taq, Polimerasa termoestable, Enzima para PCR
• French: ADN polymérase Taq, Polymérase thermostable, Enzyme pour PCR
• German: Taq-DNA-Polymerase, Thermostabile Polymerase, PCR-Enzym
• Italian: DNA polimerasi Taq, Polimerasi termostabile, Enzima per PCR

1. What is Taq DNA Polymerase used for?
Taq DNA Polymerase is widely used for PCR amplification of DNA. It enables applications such as cloning, genotyping, colony PCR, and routine DNA amplification in molecular biology workflows.

2. Does Taq DNA Polymerase have proofreading activity?
No, standard Taq DNA Polymerase lacks 3’→5’ exonuclease (proofreading) activity. This means it has a higher error rate compared to high-fidelity polymerases, but it provides robust and efficient amplification.

3. How long DNA fragments can Taq DNA Polymerase amplify?
Taq DNA Polymerase is typically suitable for amplifying DNA fragments up to 3–5 kb. For longer or more complex targets, a high-fidelity or long-range DNA polymerase is recommended.