Horse-Power™ Taq DNA Polymerase

Name: Horse-Power™ Taq DNA Polymerase
SKU: P0023
Price: 42.63 EUR
Availability: InStock

Horse-Power™ Taq DNA Polymerase is High-purity thermostable Taq polymerase for reliable, high-yield PCR amplification.

Horse-Power™ Taq DNA Polymerase is a versatile, thermostable, recombinant enzyme designed for routine PCR applications.
Produced in a genetically engineered E. coli strain expressing the Thermus aquaticus DNA polymerase I gene, the enzyme provides high activity, exceptional purity, and proven thermostability.

It exhibits 5’→3’ polymerase and weak 5’→3’ exonuclease activity, but lacks 3’→5’ exonuclease (proofreading) function.
This makes it ideal for standard amplifications and TA-cloning workflows.

IVD-grade reagent also available Ask us for regulatory formats and bulk requests.

Have questions? Contact us

Price: 42.63 €

SKU: P0023 Categories: End Point PCR, DNA Polymerases

Detailed information:

Advantages & Features

Highest purity: > 98 % (SDS-PAGE confirmed).
High performance: excellent activity, specificity, and thermostability.
Efficient reactivation buffer: optimized for reproducible PCR results
Thermostable: half-life ≈ 40 min at 94 °C.
A-tailing activity: adds 3′-adenine overhangs for TA cloning.
Incorporates modified nucleotides: biotinylated, fluorescent, etc.
Molecular weight: 94 kDa.
Complete solution: includes MgCl₂ for immediate PCR setup.

Specifications

Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl₂, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

Includes

Includes for 500 U:
– 100 μL Horse-Power™ Taq Polymerase (5 U/μL)
– 25 mM MgCl₂ (1.5 mL)
– 1.5 mL Buffer (10x)

Download documentation

Datasheet

MSDS

Brochure

Applications

Routine amplifications.
Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).

Tables & Figures

Quality Control

Functionally tested in PCR.
Undetected bacterial DNA (by PCR).
Undetectable nucleases (endo-, exo, and ribonucleases) activities guaranteed by appropriate quality tests.

Advice

Storage, Shipping & Guarantee

Shipped in: Gel pack.
Storage: -20 ºC (NON Frost-Free Freezer).

Citations

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This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.