HotBegan™ Hot Start Taq-DNA Polymerase

– 100 μL HotBegan™ Hot Start Taq DNA Polymerase (5 U/μL)
– 25 mM MgCl₂ (1.5 mL)
– 1.5 mL Buffer (10x)

• High-specificity PCR amplification.

• Multiplex and diagnostic PCR assays.

• Low-copy target amplification.

• qPCR and endpoint PCR applications.

• Routine PCR with complex DNA templates.

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• Each batch is tested for specificity, thermostability, fidelity, and absence of DNase/RNase contamination, ensuring reproducible high-quality PCR results.

• Include an initial 95 °C activation step (2–10 min) before cycling.

• Use optimized annealing temperatures for complex templates.

• Store aliquots to avoid repeated freeze–thaw cycles.

• Shipped in: Gel pack.
• Storage: -20 °C.

• Mora-Sala, B., León, M., Pérez-Sierra, A., & Abad-Campos, P. (2022). New Reports of Phytophthora Species in Plant Nurseries in Spain. Pathogens, 11(8), 826.
• Aigoun-Mouhous, Wassila, et al. “Cadophora sabaouae sp. nov. and Phaeoacremonium species associated with Petri disease on grapevine propagation material and young grapevines in Algeria.” Plant Disease ja (2021).
• León, Maela, et al. “Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain.“ Agronomy 10.8 (2020): 1062.
• Albuquerque, C. A. D. (2019). Detection and characterization of Dichelobacter nodosus from sheep with different clinical manifestations of Ovine Footrot (Doctoral dissertation).
• Roque, J. M. L. (2019). Mecanismos virucidas da irradiação por feixe de eletrões em adenovírus humanos (Doctoral dissertation).
• Elena, G., Berbegal, M., González-Domínguez, E., & Armengol, J. (2019). Identification of inoculum sources of Fusicladium eriobotryae in loquat orchards in Spain. European Journal of Plant Pathology, 1-12.
• Osset Iborra, H. (2019). Seguimiento de las plagas de Tomicus destruens y Orthotomicus erosus y detección de Botryosphaeriaceae, hongos patógenos asociados a estos insectos, en los pinares de Pinus halepensis de La Pedrera y la Devesa del Saler (Valencia) (Doctoral dissertation).
• Olmo, D., Armengol, J., León, M., & Gramaje, D. (2016). Characterization and pathogenicity of Botryosphaeriaceae species isolated from almond trees on the island of Mallorca (Spain). Plant disease, 100(12), 2483-2491.
• Negreiros, A. M., Júnior, R. S., Rodrigues, A. P., León, M., & Armengol, J. Prevalent weeds collected from cucurbit fields in Northeastern Brazil reveal new species diversity in the genus Monosporascus. Annals of Applied Biology.
• Martín-Pinto, P. Fungal communities from forest systems in Ethiopia.
• Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession under Eucalyptus grandis plantations in Ethiopia. Forest Ecology and Management, 405, 179-187.
• Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession following stand development in Pinus patula Schiede ex Schltdl. & Cham. plantations in Ethiopia. Forest Ecology and Management, 395, 9-18.
• Huyghe, J. (2017). Selection of reference genes for gene expression studies in Lactic acid bacteria.
• Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal community succession and sporocarp production following fire occurrence in Dry Afromontane forests of Ethiopia. Forest Ecology and Management, 398, 37-47.
• Criado-García, J., Fuentes, F., Cruz-Teno, C., García-Rios, A., Jiménez-Morales, A., Delgado-Lista, J., … & Pérez-Jiménez, F. (2011). R353Q polymorphism in the factor VII gene and cardiovascular risk in Heterozygous Familial Hypercholesterolemia: a case-control study. Lipids in health and disease, 10(1), 50.
• Català, S., Pérez-Sierra, A., & Abad-Campos, P. (2015). The use of genus-specific amplicon pyrosequencing to assess Phytophthora species diversity using eDNA from soil and water in northern Spain. PloS one, 10(3), e0119311.
• Olmo, D., Armengol, J., León, M., & Gramaje, D. (2016). Characterization and Pathogenicity of Botryosphaeriaceae Species Isolated from Almond Trees on the Island of Mallorca (Spain). Plant Disease, 100(12), 2483-2491.
• Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession following stand development in Pinus patula Schiede ex Schltdl. & Cham. plantations in Ethiopia. Forest Ecology and Management, 395, 9-18.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What is the activation temperature for HotBegan™ Hot-Start Taq?
The enzyme activates fully at 95 °C during the initial denaturation step.

Q2: Can it be used for multiplex PCR?
Yes. It provides excellent performance and specificity in multiplex assays.

Q3: Does the enzyme require special buffers?
No. It comes with an optimized reaction buffer suitable for most PCR applications.