Horse-Power™ Green-Taq DNA Polymerase, MasterMix

Name: Horse-Power™ Green-Taq DNA Polymerase, MasterMix
SKU: P0029-S
Price: 42.76 EUR
Availability: InStock

Ready-to-use 2× PCR MasterMix with green loading dye for fast, reproducible amplification

Horse-Power™ Green Taq DNA Polymerase MasterMix is a convenient, ready-to-use solution designed to simplify standard PCR reactions. It contains Canvax’s reliable Taq DNA Polymerase, optimized reaction buffer, MgCl₂, dNTPs, and green loading dyes for direct gel electrophoresis after amplification.

This 2× MasterMix minimizes pipetting steps, reduces contamination risk, and ensures consistent yields and reproducibility. It is ideal for routine PCR, colony screening, genotyping, and fragment analysis, offering strong amplification efficiency and time-saving convenience for everyday molecular workflows.

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100 rxn

Price: 42.76 €

SKU: P0029-S Categories: End Point PCR, MasterMixes

Detailed information:

Advantages & Features

Ready-to-use format: Just add template and primers.
Integrated loading dye: Directly load PCR products onto agarose gels.
High performance: Reliable amplification across a broad range of templates.
Reduced variability: Pre-optimized formulation ensures reproducibility.
Time-saving workflow: Minimizes setup and pipetting errors.
Convenient visualization: Includes tracking dyes for quick gel confirmation.

Specifications

Assay conditions:
Enzyme activity is assayed in the following mixture: 25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl₂, 0.1 mg/mL gelatine, 200 µM de dATP, dGTP, dTTP, 100 µM* α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

Concentration:
2x (Buffer Green 2X; TruePure™ dNTPs 0.4 mM each; Horse-Power™ Taq DNA Polymerase 0.2 U/μL, Glycerol 24%).

Includes

– 100 rxn Horse-Power™ Green-Taq DNA Polymerase MasterMix (2x)

*Includes all necessary reagents except template and primer DNA.

Download documentation

Datasheet

MSDS

Applications

Routine PCR and DNA amplification.
Colony screening and genotyping.
DNA fragment analysis and cloning.
Educational and diagnostic research workflows.
Direct agarose gel loading.

Tables & Figures

Quality Control

Each batch is tested for activity, specificity, yield consistency, and absence of DNase/RNase contamination to ensure reliable PCR performance.

Advice

Mix gently before use; avoid vortexing.
Use 10–100 ng DNA template per reaction.
For difficult templates, optimize annealing temperature or Mg²⁺ concentration.

Storage, Shipping & Guarantee

Shipped in: Gel pack.
Storage: -20 °C.

Citations

Peykov, S., Strateva, T., & Dimov, S. (2022). Design of Species-Specific PCR Primers That Target the aac (6′)-Ii Gene for the Rapid Detection of Enterococcus faecium. Bacteria, 1(3), 183-190.
Almón, B., Pérez-Dieste, J., de Carlos, A., & Bañón, R. (2022). Identification of the shell-boring parasite Polydora hoplura (Annelida: Spionidae) on wild stocks of Pecten maximus in Galician waters, NW Spain. Journal of Invertebrate Pathology, 107750.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

FAQs

Q1: Can the mix be loaded directly on gels?
Yes. The formulation includes green loading dyes for direct electrophoresis without adding loading buffer.

Q2: Is it compatible with colony PCR?
Yes. It provides robust amplification from bacterial colonies or crude extracts.

Q3: What fragment size range can it amplify?
Up to 5 kb with standard templates under optimized conditions.