T4 DNA Ligase

Name: T4 DNA Ligase
SKU: CL006
Price: 118.52 EUR
Availability: InStock

Highly efficient enzyme for ligation of cohesive and blunt DNA ends.

T4 DNA Ligase from Canvax™ is a high-efficiency enzyme that catalyzes the formation of phosphodiester bonds between adjacent 5′-phosphate and 3′-hydroxyl termini in double-stranded DNA or RNA. It is ideal for molecular cloning, linker ligation, and nick repair during DNA replication or repair processes. The enzyme works efficiently with both sticky and blunt-ended DNA fragments, delivering high ligation yields and reproducible results.

Produced under ISO-certified quality standards, Canvax™ T4 DNA Ligase ensures purity, stability, and consistent performance across different cloning applications. It provides reliable results even with low DNA concentrations and is compatible with most restriction enzyme buffers. Each batch undergoes extensive quality control to guarantee activity and integrity, making it an essential tool for routine and advanced molecular biology workflows.

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1000 U

Price: 118.52 €

SKU: CL006 Categories: End Point PCR, Related Enzymes & Products

Detailed information:

Advantages & Features

High efficiency: ligates both cohesive and blunt DNA ends.
Versatile applications: suitable for cloning, library prep, and recombinant DNA.
Reliable performance: consistent activity validated per lot.
User-friendly: supplied in ready-to-use concentration.
Trusted tool: widely applied in molecular biology workflows.

Specifications

Unit Definition:
One Weiss unit of the enzyme catalyses the conversion of 1 nmol of [32PPi] into Norit- adsorbable form in 20 min at 37 ºC. One Weiss unit is equivalent to approximately 200 cohesive end ligation (CEL) units and one CEL unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of 1 μg lambda DNA in 30 min. at 16 ºC.

Includes

– 200 µL T4 DNA Ligase (5 U Weiss/µL)
– 1.5 mL T4 DNA Ligase Buffer (10x)

Download documentation

Datasheet

MSDS

Applications

Molecular cloning.
DNA library preparation.
Recombinant DNA construction.
Vector-insert ligation.

Tables & Figures

Quality Control

Each lot is tested for activity with both sticky and blunt-end ligation, ensuring reproducibility and absence of contaminating nucleases.

Advice

For blunt-end ligations, use higher enzyme concentrations or longer incubation times.
Store enzyme in small aliquots to avoid repeated freeze-thaw cycles.
Ensure DNA fragments have 5′ phosphate groups for ligation efficiency.

Storage, Shipping & Guarantee

Shipped in: Gel pack.
Storage: -20 °C.

Citations

Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
Jiménez Muñoz, R. (2020). Desarrollo de herramientas fisiológicas y genómicas para mejorar la calidad postcosecha del fruto de calabacín.
Lago-Docampo, M., Tenorio, J., Hernández-González, I., Pérez-Olivares, C., Escribano-Subías, P., Pousada, G., … & Valverde, D. (2020). Characterization of rare ABCC8 variants identified in Spanish pulmonary arterial hypertension patients. Scientific Reports, 10(1), 1-13.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.