T7 Fluorescein RNA Labeling Kit
High-efficiency fluorescein RNA labeling kit for in vitro transcription using T7 RNA polymerase.
T7 Fluorescein RNA Labeling Kit provides a fast and reliable method for the in vitro synthesis and fluorescent labeling of RNA using T7 RNA polymerase. The system incorporates fluorescein-labeled nucleotides during transcription, producing high-quality, fluorescently tagged RNA suitable for sensitive detection in hybridization or imaging assays.
This ready-to-use formulation ensures consistent labeling efficiency and transcription yield. It is ideal for probe generation, RNA localization, microarray, and in situ hybridization experiments, offering reproducible fluorescence intensity and high signal-to-noise ratios.
Have questions? Contact us
Detailed information:
Advantages & Features
-
Integrated labeling system: Combines transcription and fluorescein incorporation in one step.
-
High sensitivity: Generates bright, detectable RNA probes for precise signal quantification.
-
Ready-to-use reagents: Minimizes setup time and technical variability.
-
Optimized T7 enzyme system: Ensures efficient incorporation of labeled nucleotides.
-
Consistent performance: Produces reproducible yields across multiple templates.
-
Compatible templates: Works with linearized plasmids or PCR DNA containing a T7 promoter.
Specifications
Includes
– 2 x 40 μl T7 RNA Polymerase mix (include RNase inhibitor)
– 200 μl T7 Reaction Buffer 10x (HEPES-based)
– 100 μl ATP (100 mM)
– 100 μl GTP (100 mM) 100 μl
– 100 μl CTP (100 mM) 100 μl
– 100 μl UTP (100 mM) 100 μl
– 10 μl Fluorescein-12-UTP (5mM)
– 100 μl DTT (100 mM)
– 10 µl Control template 1 (200 ng/µl)
– 1.2 ml Nuclease-Free Water
Download documentation
Datasheet MSDSApplications
-
In situ hybridization (ISH).
-
Microarray and blot hybridization.
-
RNA probe labeling for Northern blotting.
-
RNA localization and imaging assays.
-
Fluorescent RNA tracking studies.
Tables & Figures
Quality Control
- Each batch is tested for transcription yield, labeling efficiency, and RNase-free integrity to guarantee consistent fluorescence intensity and RNA quality.
Advice
-
Use high-purity, linearized DNA as a transcription template.
-
Protect samples from light during and after labeling.
-
Keep reagents cold and use RNase-free consumables.
-
Verify incorporation by measuring fluorescence emission at 520 nm.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What type of fluorophore is incorporated?
Fluorescein-UTP is integrated during transcription for direct RNA labeling.
Q2: Can this kit be used with other RNA synthesis kits?
Yes. It is compatible with templates used in CVX™ T7, SP6, or T3 RNA Synthesis Kits.
Q3: How is labeling efficiency verified?
Labeling can be confirmed by fluorescence measurement or gel imaging at 520 nm.
